Fate of microtubule-organizing centers during myogenesis in vitro.
Fate of microtubule-organizing centers during myogenesis in vitro.
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DOI：
10.1083/jcb.100.1.35
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发表时间：
1985-01
期刊：
影响因子：
--
通讯作者：
Bornens M
中科院分区：
文献类型：
--
作者：
Tassin AM;Maro B;Bornens M
微管组织和成核研究在体外人肌形成的免疫细胞学，使用单克隆和多克隆抗微管蛋白抗体和兔非免疫血清，与人中心体反应。在成肌细胞中，我们观察到一个经典的微管网络为中心的非核中心体。肌管具有许多平行排列的微管，没有任何明显的成核中心。这些细胞中的中心体并不与每个细胞核相关联，但通常聚集在核群附近。它们明显小于单核细胞。用标记中心体的血清标记肌管中每个核的外围，表明微管成核物质的深刻重组。诺考达唑处理后的再生长实验确定微管从细胞核的周边生长。成核物质的重新分布发生在成肌细胞融合后早期。这种现象似乎是特定的肌细胞分化的人工诱导的多核体表现不同：中心体聚集形成只有一个或几个巨大的成核中心和核不直接参与微管的成核。这些结果的意义进行了讨论有关的中心体在建立细胞极性的可能作用。
Microtubule organization and nucleation were studied during in vitro human myogenesis by immunocytology that used monoclonal and polyclonal antitubulin antibodies and a rabbit nonimmune serum that reacts with human centrosomes. In myoblasts, we observed a classical microtubule network centered on juxtanuclear centrosomes. Myotubes possessed numerous microtubules organized in parallel without any apparent nucleation centers. Centrosomes in these cells were not associated one to each nucleus but were often clustered in the vicinity of nuclei groups. They were significantly smaller than those of the mononucleated cells. The periphery of each nucleus in myotubes was labeled with the serum that labels centrosomes suggesting a profound reorganization of microtubule-nucleating material. Regrowth experiments after Nocodazole treatment established that microtubules were growing from the periphery of the nuclei. The redistribution of nucleating material was shown to take place early after myoblast fusion. Such a phenomenon appears to be specific to myogenic differentiation in that artificially induced polykaryons behaved differently: the centrosomes aggregated to form only one or a few giant nucleating centers and the nuclei did not participate directly in the nucleation of microtubules. The significance of these results is discussed in relation to the possible role of the centrosome in establishing cell polarity.
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影响因子：
--
作者：
ALBRECHTBUEHLER, G
通讯作者：
ALBRECHTBUEHLER, G
影响因子：
64.8
作者：
MARO, B;BORNENS, M
通讯作者：
BORNENS, M
影响因子：
7.8
作者：
KURIYAMA, R;BORISY, GG
通讯作者：
BORISY, GG
DOI：
10.1083/jcb.75.3.666
发表时间：
1977-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Malech HL;Root RK;Gallin JI
通讯作者：
Gallin JI
影响因子：
0.6
作者：
DEBRABANDER, M
通讯作者：
DEBRABANDER, M