Nonrandom distribution of interhomolog recombination events induced by breakage of a dicentric chromosome in Saccharomyces cerevisiae.
Nonrandom distribution of interhomolog recombination events induced by breakage of a dicentric chromosome in Saccharomyces cerevisiae.
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酿酒酵母中二十分染色体破裂引起的非随机重组事件的非随机分布。
DOI：
10.1534/genetics.113.150144
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发表时间：
2013-05
期刊：
影响因子：
3.3
通讯作者：
Petes TD
中科院分区：
文献类型：
--
作者：
Song W;Gawel M;Dominska M;Greenwell PW;Hazkani-Covo E;Bloom K;Petes TD
双着丝粒染色体在有丝分裂中发生断裂，导致染色体缺失、重复和易位。在这项研究中，我们地图的双着丝粒染色体断裂位点的酿酒酵母有丝分裂重组试验。该测定使用二倍体菌株，其中一个同源物除了野生型着丝粒外还具有条件性着丝粒，另一个同源物仅具有野生型着丝粒;当细胞在半乳糖中生长时，条件性着丝粒是无活性的，当细胞转换为葡萄糖时，条件性着丝粒被激活。此外，这两种同源物可通过多个单核苷酸多态性（SNP）来区分。在条件性着丝粒被激活的条件下，功能性双着丝粒染色体经历双链DNA断裂（DSB），其可以通过与同源物的有丝分裂重组来修复。这样的重组事件经常导致在交叉的着丝粒远端的SNP的杂合性丢失（洛）。使用基于PCR的分析，我们确定了洛缺失在多个独立重组事件中的位置，分辨率为1.4kb。该分析表明，双着丝粒染色体具有广泛分布在两个着丝粒之间的重组断裂点，尽管在条件性着丝粒的10 kb内存在断裂点的聚类。
Dicentric chromosomes undergo breakage in mitosis, resulting in chromosome deletions, duplications, and translocations. In this study, we map chromosome break sites of dicentrics in Saccharomyces cerevisiae by a mitotic recombination assay. The assay uses a diploid strain in which one homolog has a conditional centromere in addition to a wild-type centromere, and the other homolog has only the wild-type centromere; the conditional centromere is inactive when cells are grown in galactose and is activated when the cells are switched to glucose. In addition, the two homologs are distinguishable by multiple single-nucleotide polymorphisms (SNPs). Under conditions in which the conditional centromere is activated, the functionally dicentric chromosome undergoes double-stranded DNA breaks (DSBs) that can be repaired by mitotic recombination with the homolog. Such recombination events often lead to loss of heterozygosity (LOH) of SNPs that are centromere distal to the crossover. Using a PCR-based assay, we determined the position of LOH in multiple independent recombination events to a resolution of ∼4 kb. This analysis shows that dicentric chromosomes have recombination breakpoints that are broadly distributed between the two centromeres, although there is a clustering of breakpoints within 10 kb of the conditional centromere.
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影响因子：
10.5
作者：
Admire, A;Shanks, L;Weinert, T
通讯作者：
Weinert, T
影响因子：
64.5
作者：
Blat, Y;Kleckner, N
通讯作者：
Kleckner, N
影响因子：
8.6
作者：
BENSIMON, D;SIMON, AJ;BENSIMON, A
通讯作者：
BENSIMON, A
影响因子：
9.8
作者：
Glynn, EF;Megee, PC;Gerton, JL
通讯作者：
Gerton, JL
DOI：
10.1073/pnas.0913883107
发表时间：
2010-01-26
影响因子：
11.1
作者：
Gresham, David;Curry, Bo;Botstein, David
通讯作者：
Botstein, David