Isolation and Identification of a Recombinant Porcine Epidemic Diarrhea Virus With a Novel Insertion in S1 Domain.
Isolation and Identification of a Recombinant Porcine Epidemic Diarrhea Virus With a Novel Insertion in S1 Domain.
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DOI：
10.3389/fmicb.2021.667084
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发表时间：
2021
影响因子：
5.2
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Li D;Li Y;Liu Y;Chen Y;Jiao W;Feng H;Wei Q;Wang J;Zhang Y;Zhang G
猪流行性腹泻病毒（Porcine epidemic diarrhea virus，PEDV）是引起新生仔猪腹泻和高死亡率的主要病原，对养猪业造成严重的影响。病毒变异和变异是PEDV进化和遗传多样性的主要驱动力。2016年，华中地区一家集约化猪场发生仔猪腹泻疫情。从临床样品中成功分离出一种新的PEDV分离株（称为HNAY）。序列分析表明，HNAY的S1基因存在21个核苷酸的插入，这在其它PEDV分离物中未见报道。该插入片段的序列与PEDV N基因中的序列片段完全一致。值得注意的是，HNAY毒株在S1蛋白的中和表位中表现出两个独特的突变（T500A和L521Y），其不同于其他PEDV变体毒株和基于CV777的疫苗毒株的那些。此外，PEDV HNAY可能来源于两个中国变异株PEDV之间的天然重组。动物实验表明，HNAY与其他两株临床分离株相比，具有较高的致病性。本研究为进一步了解PEDV的遗传进化和分子致病机制奠定了基础。
Porcine epidemic diarrhea virus (PEDV) is the major pathogen that causes diarrhea and high mortality in newborn piglets with devastating impact to the pig industry. Recombination and mutation are the main driving forces of viral evolution and genetic diversity of PEDV. In 2016, an outbreak of diarrhea in piglets occurred in an intensive pig farm in Central China. A novel PEDV isolate (called HNAY) was successfully isolated from clinical samples. Sequence analysis and alignment showed that HNAY possessed 21-nucleotide (nt) insertion in its S1 gene, which has never been reported in other PEDV isolates. Moreover, the sequence of the insertion was identical with the sequence fragment in PEDV N gene. Notably, the HNAY strain exhibited two unique mutations (T500A and L521Y) in the neutralizing epitopes of the S1 protein that were different from those of other PEDV variant strains and CV777-based vaccine strains. Additionally, PEDV HNAY might be derived from a natural recombination between two Chinese variant PEDV strains. Animal experiments demonstrated that HNAY displayed higher pathogenicity compared with two other clinical isolates. This study lays the foundation for better understanding of the genetic evolution and molecular pathogenesis of PEDV.
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