Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato.
Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato.
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DOI：
10.1093/jxb/eru448
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发表时间：
2015-02
影响因子：
6.9
通讯作者：
Roitsch T
中科院分区：
文献类型：
--
作者：
Albacete A;Cantero-Navarro E;Großkinsky DK;Arias CL;Balibrea ME;Bru R;Fragner L;Ghanem ME;González Mde L;Hernández JA;Martínez-Andújar C;van der Graaff E;Weckwerth W;Zellnig G;Pérez-Alfocea F;Roitsch T
细胞壁转化酶基因的过表达通过限制气孔导度、诱导源叶片的汇代谢和光合作用，促进番茄的脱水避免和水分利用效率，延缓衰老。干旱胁迫条件改变源库关系，从而影响植物生长、适应性反应，进而影响作物产量。转化酶是通过将蔗糖水解裂解为己糖单体来调节汇活性的关键代谢酶，在植物生长发育中起着至关重要的作用。然而，转化酶在适应非生物应激条件中的生理作用尚未完全了解。研究表明，过表达红藜（Chenopodium rubrum）细胞壁转化酶（cwInv）基因CIN1可显著提高番茄（Solanum lycopersicum L.）对干旱胁迫的适应性。在正常浇水条件下，CIN1过表达限制了气孔导度，导致干旱时期耗水量减少，而光合活性保持不变。这导致水分利用效率大幅提高（高达50%），通过有效的脱水避免生理策略显著提高了水分胁迫适应能力。干旱胁迫显著降低cwInv活性，诱导cwInv蛋白抑制剂在野生型植物叶片中表达。然而，在所有分析条件下，过表达cin1的植株的cwInv活性高出3- 6倍。令人惊讶的是，由于代谢碳水化合物通量的激活，转化酶活性的增强并没有导致己糖浓度的增加，这反映在初级代谢关键酶的活性维持和缺水条件下糖磷酸盐中间体水平的增加上。干旱胁迫下，诱导叶片汇代谢可以维持光合活性，延缓衰老，提高源活性。此外，CIN1植株在活性氧产生和持续膜保护方面也表现出较好的控制能力。这些由CIN1过表达引起的代谢变化伴随着叶片中延缓衰老激素反式玉米素浓度的增加和诱导衰老的乙烯前体1-氨基环丙烷-1-羧酸（ACC）浓度的降低。因此，cwInv在代谢、激素和胁迫信号的整合点起着关键作用，为克服干旱对作物产量的限制提供了一种新的策略，而不会对最佳生长条件下的植物适应性产生负面影响。
Overexpression of a cell wall invertase gene promotes dehydration avoidance and WUE, and delays senescence in tomato through limiting stomatal conductance and inducing sink metabolism and photosynthesis in source leaves. Drought stress conditions modify source–sink relations, thereby influencing plant growth, adaptive responses, and consequently crop yield. Invertases are key metabolic enzymes regulating sink activity through the hydrolytic cleavage of sucrose into hexose monomers, thus playing a crucial role in plant growth and development. However, the physiological role of invertases during adaptation to abiotic stress conditions is not yet fully understood. Here it is shown that plant adaptation to drought stress can be markedly improved in tomato (Solanum lycopersicum L.) by overexpression of the cell wall invertase (cwInv) gene CIN1 from Chenopodium rubrum. CIN1 overexpression limited stomatal conductance under normal watering regimes, leading to reduced water consumption during the drought period, while photosynthetic activity was maintained. This caused a strong increase in water use efficiency (up to 50%), markedly improving water stress adaptation through an efficient physiological strategy of dehydration avoidance. Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants. However, the CIN1-overexpressing plants registered 3- to 6-fold higher cwInv activity in all analysed conditions. Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation. The induced sink metabolism in the leaves explained the maintenance of photosynthetic activity, delayed senescence, and increased source activity under drought stress. Moreover, CIN1 plants also presented a better control of production of reactive oxygen species and sustained membrane protection. Those metabolic changes conferred by CIN1 overexpression were accompanied by increases in the concentrations of the senescence-delaying hormone trans-zeatin and decreases in the senescence-inducing ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in the leaves. Thus, cwInv critically functions at the integration point of metabolic, hormonal, and stress signals, providing a novel strategy to overcome drought-induced limitations to crop yield, without negatively affecting plant fitness under optimal growth conditions.
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影响因子：
6.2
作者：
Fan, Wei;Zhang, Zhili;Zhang, Yanlin
通讯作者：
Zhang, Yanlin
影响因子：
7.4
作者：
Grosskinsky, Dominik K.;Naseem, Muhammad;Roitsch, Thomas
通讯作者：
Roitsch, Thomas
影响因子：
4.3
作者：
Ben Salah, Imene;Albacete, Alfonso;Abdelly, Chedly
通讯作者：
Abdelly, Chedly
影响因子：
27.5
作者：
Bonfig, Katharina B.;Gabler, Andrea;Roitsch, Thomas
通讯作者：
Roitsch, Thomas
影响因子：
5.1
作者：
ELLIOTT, KJ;BUTLER, WO;MIRKOV, TE
通讯作者：
MIRKOV, TE