Understanding the impact of bioactive coating materials for human mesenchymal stromal cells and implications for manufacturing.
Understanding the impact of bioactive coating materials for human mesenchymal stromal cells and implications for manufacturing.
复制标题
DOI：
10.1007/s10529-023-03369-9
复制
发表时间：
2023-08
影响因子：
2.7
通讯作者：
Rafiq, Qasim A.
中科院分区：
文献类型：
--
作者：
Couto, Pedro Silva;Molina, Samuel A.;O'Sullivan, Denis;O'Neill, Liam;Lyness, Alexander M.;Rafiq, Qasim A.
生物活性材料与细胞相互作用并调节其特性，从而能够产生具有所需规格的基于细胞的产品。然而，在建立细胞疗法制造工艺时，它们的评估和影响往往被忽视。在这项研究中，我们研究了不同的组织培养表面，包括未经处理的聚苯乙烯表面，未涂覆的环烯烃聚合物（COP）和COP与胶原蛋白和重组纤连蛋白包被的作用。据观察，人间充质基质细胞（hMSC）扩大COP包被板与不同的生物活性材料导致改善细胞生长动力学相比，传统的聚苯乙烯板和非包被的COP板。对于接种在用I型胶原和重组纤连蛋白包被的COP板中的hMSC，获得的倍增时间分别为2.78和3.02天，而对于接种在标准聚苯乙烯处理的板中的细胞，获得的倍增时间为4.64天。代谢物分析加强了生长动力学研究的结果，特别是在胶原蛋白I和纤连蛋白包被的COP平板上培养的细胞显示出生长改善，与聚苯乙烯组的细胞（5.86 × 105 pmol/细胞/天）相比，乳酸产生率更高（分别为9.38 × 105和9.67 × 105 pmol/细胞/天）证明了这一点。这项研究表明，COP是一种有效的替代聚苯乙烯处理的板时，涂覆有生物活性材料，如胶原蛋白和纤连蛋白，但COP处理的板没有额外的涂层被发现不足以支持细胞生长。这些发现证明了生物材料在细胞制造过程中的关键作用以及优化这种选择的重要性。
Bioactive materials interact with cells and modulate their characteristics which enable the generation of cell-based products with desired specifications. However, their evaluation and impact are often overlooked when establishing a cell therapy manufacturing process. In this study, we investigated the role of different surfaces for tissue culture including, untreated polystyrene surface, uncoated Cyclic Olefin Polymer (COP) and COP coated with collagen and recombinant fibronectin. It was observed that human mesenchymal stromal cells (hMSCs) expanded on COP-coated plates with different bioactive materials resulted in improved cell growth kinetics compared to traditional polystyrene plates and non-coated COP plates. The doubling time obtained was 2.78 and 3.02 days for hMSC seeded in COP plates coated with collagen type I and recombinant fibronectin respectively, and 4.64 days for cells plated in standard polystyrene treated plates. Metabolite analysis reinforced the findings of the growth kinetic studies, specifically that cells cultured on COP plates coated with collagen I and fibronectin exhibited improved growth as evidenced by a higher lactate production rate (9.38 × 105 and 9.67 × 105 pmol/cell/day, respectively) compared to cells from the polystyrene group (5.86 × 105 pmol/cell/day). This study demonstrated that COP is an effective alternative to polystyrene-treated plates when coated with bioactive materials such as collagen and fibronectin, however COP-treated plates without additional coatings were found not to be sufficient to support cell growth. These findings demonstrate the key role biomaterials play in the cell manufacturing process and the importance of optimising this selection.
登录
查看更多内容
影响因子：
4.5
作者：
Chen, Allen Kuan-Liang;Chew, Yi Kong;Oh, Steve Kah Weng
通讯作者：
Oh, Steve Kah Weng
影响因子：
3.9
作者：
Dudek, Magdalena M.;Gandhiraman, R. P.;Killard, Anthony J.
通讯作者：
Killard, Anthony J.
影响因子：
3.3
作者：
de Soure, Antonio M.;Fernandes-Platzgummer, Ana;da Silva, Claudia L.
通讯作者：
da Silva, Claudia L.
膨胀培养基和纤连蛋白涂层对人骨髓间充质基质细胞的生长和软骨分化的影响。
影响因子：
4.6
作者：
Basoli V;Della Bella E;Kubosch EJ;Alini M;Stoddart MJ
通讯作者：
Stoddart MJ
影响因子：
11.8
作者：
Hosios AM;Hecht VC;Danai LV;Johnson MO;Rathmell JC;Steinhauser ML;Manalis SR;Vander Heiden MG
通讯作者：
Vander Heiden MG