Effect of expansion media and fibronectin coating on growth and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells.
Effect of expansion media and fibronectin coating on growth and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells.
复制标题
膨胀培养基和纤连蛋白涂层对人骨髓间充质基质细胞的生长和软骨分化的影响。
DOI：
10.1038/s41598-021-92270-4
复制
发表时间：
2021-06-22
影响因子：
4.6
通讯作者：
Stoddart MJ
中科院分区：
文献类型：
--
作者：
Basoli V;Della Bella E;Kubosch EJ;Alini M;Stoddart MJ
在再生医学领域，从技术和生物学的角度来看，已经取得了相当大的进展。然而，关于将基于间充质基质细胞（MSC）的疗法转化和应用于临床实践，仍有很大的空白有待填补。事实上，诸如细胞类型、不可预测的供体变异和扩增/分化方法等变量导致不一致。大多数方案在MSC扩增期间使用牛血清（FBS）衍生物。然而，与FBS相关的异种风险限制了基于MSC的产品在临床实践中的使用。在此，我们比较了化学成分确定的、无异种的商业生长培养基与含有10%FBS和5 ng/ml FGF 2的常规培养基。此外，纤连蛋白包被的生长表面的效果进行了研究。通过分析基质沉积和常见软骨形成标志物的基因表达来评估不同培养条件对软骨形成承诺的影响。含FBS的αMEM和具有纤连蛋白涂层的化学成分确定的培养基之间的软骨形成分化潜力相似。相反，使用含有FBS的培养基的纤连蛋白涂层似乎降低了MSC的分化潜力。此外，对体外软骨形成刺激反应差的细胞在含有TGF-β1的培养基中扩增后显示出提高其分化潜力。总之，由于保持细胞特性和效力的能力，使用无异种的培养基为人骨髓MSC扩增提供了合适的替代方案。为了进一步提高BMSCs的成软骨潜能，在扩增过程中用TGF-β1预处理细胞是一种有前途的策略。
In the field of regenerative medicine, considerable advances have been made from the technological and biological point of view. However, there are still large gaps to be filled regarding translation and application of mesenchymal stromal cell (MSC)-based therapies into clinical practice. Indeed, variables such as cell type, unpredictable donor variation, and expansion/differentiation methods lead to inconsistencies. Most protocols use bovine serum (FBS) derivatives during MSC expansion. However, the xenogeneic risks associated with FBS limits the use of MSC-based products in clinical practice. Herein we compare a chemically defined, xenogeneic-free commercial growth medium with a conventional medium containing 10% FBS and 5 ng/ml FGF2. Furthermore, the effect of a fibronectin-coated growth surface was investigated. The effect of the different culture conditions on chondrogenic commitment was assessed by analyzing matrix deposition and gene expression of common chondrogenic markers. Chondrogenic differentiation potential was similar between the FBS-containing αMEM and the chemically defined medium with fibronectin coating. On the contrary, the use of fibronectin coating with FBS-containing medium appeared to reduce the differentiation potential of MSCs. Moreover, cells that were poorly responsive to in vitro chondrogenic stimuli were shown to improve their differentiation potential after expansion in a TGF-β1 containing medium. In conclusion, the use of a xenogeneic-free medium provides a suitable alternative for human bone marrow MSC expansion, due the capability to maintain cell characteristic and potency. To further improve chondrogenic potential of BMSCs, priming the cells with TGF-β1 during expansion is a promising strategy.
登录
查看更多内容
DOI：
10.3343/kjlm.2011.31.3.212
发表时间：
2011-07
期刊：
The Korean journal of laboratory medicine
影响因子：
--
作者：
Cho HS;Song IH;Park SY;Sung MC;Ahn MW;Song KE
通讯作者：
Song KE
影响因子：
5.2
作者：
Gregory, Carl A.;Reyes, Emigdio;Spees, Jeffrey L.
通讯作者：
Spees, Jeffrey L.
影响因子：
46.9
作者：
Lipsitz, Yonatan Y.;Timmins, Nicholas E.;Zandstra, Peter W.
通讯作者：
Zandstra, Peter W.
影响因子：
--
作者：
Monaco G;El Haj AJ;Alini M;Stoddart MJ
通讯作者：
Stoddart MJ
影响因子：
--
作者：
Grogan, Shawn Patrick;Barbero, Andrea;Mainil-Varlet, Pierre
通讯作者：
Mainil-Varlet, Pierre