Cytokine profiles for peripheral blood lymphocytes from patients with active pulmonary tuberculosis and healthy household contacts in response to the 30-kilodalton antigen of Mycobacterium tuberculosis.
Cytokine profiles for peripheral blood lymphocytes from patients with active pulmonary tuberculosis and healthy household contacts in response to the 30-kilodalton antigen of Mycobacterium tuberculosis.
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活动性肺结核患者和健康家庭接触者的外周血淋巴细胞对 30 千道尔顿结核分枝杆菌抗原的细胞因子谱。
DOI：
10.1128/iai.66.1.176-180.1998
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发表时间：
1998
影响因子：
3.1
通讯作者：
Sada,E
中科院分区：
文献类型：
--
作者：
Torres,M;Herrera,T;Villareal,H;Rich,EA;Sada,E
Patients with active tuberculosis (TB) have a stronger humoral but a poorer cellular immune response to the secreted 30-kDa antigen (Ag) ofMycobacterium tuberculosisthan do healthy household contacts (HHC), who presumably are more protected against disease. The basis for this observation was studied by examining the Th1 (interleukin 2 [IL-2] and gamma interferon [IFN-γ])- and Th2 (IL-10 and IL-4)-type cytokines produced in response to the 30-kDa Ag by peripheral blood mononuclear cells (PBMC) from patients with active pulmonary TB (n= 7) and from HHC who were tuberculin (purified protein derivative) skin test positive (n= 12). Thirty-kilodalton-Ag-stimulated PBMC from TB patients produced significantly lower levels of IFN-γ (none detectable) than did those from HHC (212 ± 73 pg/ml, mean ± standard error) (P< 0.001). Likewise, 30-kDa-Ag-stimulated PBMC from TB patients failed to express IFN-γ mRNA by reverse transcription-PCR, whereas cells from HHC expressed the IFN-γ gene. In contrast, 30-kDa-Ag-stimulated PBMC from TB patients produced significantly higher levels of IL-10 (403 ± 80 pg/ml) than did those from HHC (187 ± 66 pg/ml) (P< 0.013), although cells from both groups expressed the IL-10 gene. IL-2 and IL-4 were not consistently produced, and their genes were not expressed by 30-kDa-Ag-stimulated cells from either TB patients or HHC. After treatment with antituberculous drugs, lymphocytes from four of the seven TB patients proliferated and three of them expressed IFN-γ mRNA in response to the 30-kDa Ag and produced decreased levels of IL-10.
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影响因子：
4.4
作者：
P. Sieling;J. Abrams;M. Yamamura;Padmini Salgame;Barry R. Bloom;T. Rea;R. Modlin
通讯作者：
P. Sieling;J. Abrams;M. Yamamura;Padmini Salgame;Barry R. Bloom;T. Rea;R. Modlin
影响因子：
3.7
作者：
HUYGEN, K;VANVOOREN, JP;DEBRUYN, J
通讯作者：
DEBRUYN, J
DOI：
10.1590/s0042-96862001000100014
发表时间：
1991-03
期刊：
Tubercle
影响因子：
--
作者：
A. Kochi
通讯作者：
A. Kochi
BCG 故事：过去的教训和对未来的影响。
DOI：
--
发表时间：
1989
期刊：
Reviews of Infectious Diseases
影响因子：
--
作者：
P. Fine
通讯作者：
P. Fine
肺结核病程中分枝杆菌抗原复合物 A60 特异性 T 细胞库
影响因子：
3.1
作者：
S. Carlucci;A. Beschin;L. Tuosto;Franco Ameglio;G. Gandolfo;C. Cocito;F. Fiorucci;C. Saltini;E. Piccolella
通讯作者：
E. Piccolella