Cell Motility as Contrast Agent in Retinal Explant Imaging With Full-Field Optical Coherence Tomography.
Cell Motility as Contrast Agent in Retinal Explant Imaging With Full-Field Optical Coherence Tomography.
复制标题
细胞运动作为全场光学相干断层扫描视网膜外植体成像中的造影剂。
DOI：
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发表时间：
2017
影响因子：
4.4
通讯作者：
K. Grieve
中科院分区：
文献类型：
--
作者：
O. Thouvenin;C. Boccara;M. Fink;J. Sahel;M. Pâques;K. Grieve
目的
在视网膜移植中使用细胞运动作为对比剂。
方法
用高分辨率全视野光学相干断层扫描（FFOCT）和动态FFOCT结合荧光成像对猕猴和小鼠视网膜外植体进行成像。
结果
静态和动态FFOCT从细胞内的不同结构产生互补对比。在体外样本成像时，静态FFOCT检测陡峭的折射率梯度，以揭示包括纤维，血管，胶原蛋白和细胞轮廓在内的固定结构，而动态FFOCT强调主要是细胞内的移动结构的代谢活动，从而在先前隐藏在噪声中的细胞中产生或增强对比度。动态FFOCT能够检测神经节细胞、内核层和外核层中的大多数视网膜细胞类型，其中静态FFOCT对比度相对于噪声背景太低。
结论
静态和动态复合FFOCT为视网膜植块的成像提供了一种新的FFOCT图像，其中包含有价值的信息。它以亚细胞分辨率提供活体视网膜的无标记正面图像。动态FFOCT增加了有关细胞活性的信息，这在纵向外植体研究中很有意义。
Purpose
To use cell motility as a contrast agent in retinal explants.
Methods
Macaque and mouse retinal explants were imaged with high resolution full field optical coherence tomography (FFOCT) and dynamic FFOCT, coupled with fluorescence imaging.
Results
Static and dynamic FFOCT create complementary contrast from different structures within a cell. When imaging in vitro samples, static FFOCT detects steep refractive index gradients to reveal stationary structures including fibers, vessels, collagen, and cell contours, while dynamic FFOCT emphasizes metabolic activity of moving structures that are mainly intracellular, thus creating or enhancing contrast in cells that were previously hidden in noise. Dynamic FFOCT enables detection of most of the retinal cell types in the ganglion cell, inner and outer nuclear layers, where static FFOCT contrast is too low in relation to the noise background.
Conclusions
Composite static and dynamic FFOCT provides a new kind of FFOCT image containing valuable information for imaging of retinal explants. It provides label-free en face images of living retinas, with a subcellular resolution. Dynamic FFOCT adds information about cell activity, which is of interest in longitudinal explant studies.
影响因子：
16.2
作者：
Grienberger, Christine;Konnerth, Arthur
通讯作者：
Konnerth, Arthur
影响因子：
3.8
作者：
Lee, Jonghwan;Wu, Weicheng;Boas, David A.
通讯作者：
Boas, David A.
影响因子：
3.8
作者：
Jonnal RS;Besecker JR;Derby JC;Kocaoglu OP;Cense B;Gao W;Wang Q;Miller DT
通讯作者：
Miller DT