Biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells.
Biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells.
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DOI：
10.1371/journal.pone.0018738
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发表时间：
2011-04-13
期刊：
影响因子：
3.7
通讯作者：
Suh YH
中科院分区：
文献类型：
--
作者：
Chang KA;Kim JW;Kim JA;Lee SE;Kim S;Suh WH;Kim HS;Kwon S;Kim SJ;Suh YH
使用非化学方法来分化干细胞已经吸引了来自多个学科的研究人员，包括工程学和生物医学领域。毫无疑问，基于生长因子的方法仍然是实现某种程度的增殖和分化控制的最主要的方法-然而，基于化学的方法仍然受到所用试剂的质量、来源和数量的限制。干细胞分化的明确的非化学方法允许干细胞科学家通过精确地管理预定义的参数来控制干细胞生物学，无论这些参数是结构线索、底物硬度，还是以电流的形式。我们已经开发了一种培养系统，允许干细胞正常生长，并可以选择施加连续和特定水平的电流来改变正在生长的细胞的细胞生物学。这种采用ITO电极的两相电流刺激器芯片在同一培养箱中同时产生正负电流，而不影响表面化学。我们发现，双相电流(BECs)显著促进了胎儿神经干细胞(NSCs)的增殖。此外，根据免疫细胞化学的评估，BECs还促进胎儿NSCs向神经细胞的分化。我们的结果清楚地表明，BECs对胎儿神经干细胞的增殖和神经元分化都有促进作用。它可能适用于开发使用神经干细胞治疗各种神经退行性疾病的策略，如阿尔茨海默氏症和帕金森氏症。
The use of non-chemical methods to differentiate stem cells has attracted researchers from multiple disciplines, including the engineering and the biomedical fields. No doubt, growth factor based methods are still the most dominant of achieving some level of proliferation and differentiation control - however, chemical based methods are still limited by the quality, source, and amount of the utilized reagents. Well-defined non-chemical methods to differentiate stem cells allow stem cell scientists to control stem cell biology by precisely administering the pre-defined parameters, whether they are structural cues, substrate stiffness, or in the form of current flow. We have developed a culture system that allows normal stem cell growth and the option of applying continuous and defined levels of electric current to alter the cell biology of growing cells. This biphasic current stimulator chip employing ITO electrodes generates both positive and negative currents in the same culture chamber without affecting surface chemistry. We found that biphasic electrical currents (BECs) significantly increased the proliferation of fetal neural stem cells (NSCs). Furthermore, BECs also promoted the differentiation of fetal NSCs into neuronal cells, as assessed using immunocytochemistry. Our results clearly show that BECs promote both the proliferation and neuronal differentiation of fetal NSCs. It may apply to the development of strategies that employ NSCs in the treatment of various neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases.
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DOI：
10.1016/j.bbagen.2004.06.024
发表时间：
2004-11-01
影响因子：
3
作者：
Rollwitz, J;Lupke, M;Simkó, M
通讯作者：
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影响因子：
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DOI：
10.1523/jneurosci.2727-09.2009
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2009-12-02
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
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Overstreet-Wadiche LS
DOI：
10.1016/j.bbamcr.2006.06.007
发表时间：
2006-09-01
影响因子：
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Hwang, Soon Jung
DOI：
10.1016/s0165-3806(00)00069-9
发表时间：
2000-09-30
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
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