A low-cost mouse cage warming system provides improved intra-ischemic and post-ischemic body temperature control - Application for reducing variability in experimental stroke studies.
A low-cost mouse cage warming system provides improved intra-ischemic and post-ischemic body temperature control - Application for reducing variability in experimental stroke studies.
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DOI：
10.1016/j.jneumeth.2021.109228
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发表时间：
2021-08-01
影响因子：
3
通讯作者：
Marrelli SP
中科院分区：
文献类型：
--
作者：
Hong SH;Hong JH;Lahey MT;Zhu L;Stephenson JM;Marrelli SP
脑温是缺血性中风损伤的重要决定因素。为此，建议在实验性啮齿动物卒中模型中严格管理脑或体温(Tcore)，以提高结果的严密性和重复性。然而，在卒中期间和卒中后管理Tcore的方法在方法和有效性上差别很大。我们开发了一种低成本的温暖环境空气笼(WAAC)系统，以在缺血内和缺血后恢复期提供更好的温度控制。该系统被纳入标准的保持笼，用于在缺血期和恢复期的几个小时内维持Tcore。我们将WAAC系统与一种常用的热支持方法进行了比较，该方法由加热垫上的笼子组成。对两种热支持系统在小鼠大脑中动脉闭塞(MCAO)卒中模型中的作用进行了评估。WAAC系统在缺血内(60分钟)和缺血后(3小时)提供了更好的温度控制(更多的常温Tcore和更少的Tcore变化)。热支持系统组的平均脑梗塞体积无统计学差异，但WAAC系统组的标准差降低了54%。老鼠和其他小型啮齿动物在MCAO过程中和之后非常容易散热。与经常使用的感应加热方法相比，WAAC系统在接受MCAO中风模型的小鼠中提供了更精确和更可控的Tcore维护。改进的温度控制应该会提高实验的严密性，并减少所需的实验动物数量。
Brain temperature is a strong determinant of ischemic stroke injury. For this reason, tight management of brain or body temperature (Tcore) in experimental rodent stroke models is recommended to improve the rigor and reproducibility of outcomes. However, methods for managing Tcore during and after stroke vary widely in approach and effectiveness. We developed a low-cost warm ambient air cage (WAAC) system to provide improved temperature control during the intra-ischemic and post-ischemic recovery periods. The system is incorporated into standard holding cages for maintaining Tcore during the intra-ischemic period as well as for several hours into the recovery period. We compared the WAAC system with a commonly used heat support method, consisting of a cage on a heating pad. Both heat support systems were evaluated for the middle cerebral artery occlusion (MCAo) stroke model in mice. The WAAC system provided improved temperature control (more normothermic Tcore and less Tcore variation) during the intra-ischemic period (60 min) and post-ischemic period (3 hrs). Mean infarct volume was not statistically different by heat support system, however, standard deviation was 54% lower in the WAAC system group. Mice and other small rodents are highly vulnerable to heat loss during and after the MCAo procedure. The WAAC system provides more precise and controlled Tcore maintenance compared with frequently used induction heating methods in mice undergoing the MCAo stroke model. The improved temperature control should enhance experimental rigor and reduce the number of experimental animals needed.
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大脑温度稳态：生理波动和病理转移。
DOI：
10.2741/3608
发表时间：
2010-01-01
期刊：
Frontiers in bioscience (Landmark edition)
影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
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期刊：
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
影响因子：
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DOI：
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发表时间：
2014-01-01
影响因子：
2.8
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
STAIR Group