Use of Drawing Lithography-Fabricated Polyglycolic Acid Microneedles for Transdermal Delivery of Itraconazole to a Human Basal Cell Carcinoma Model Regenerated on Mice.
Use of Drawing Lithography-Fabricated Polyglycolic Acid Microneedles for Transdermal Delivery of Itraconazole to a Human Basal Cell Carcinoma Model Regenerated on Mice.
复制标题
DOI：
10.1007/s11837-016-1841-1
复制
发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Narayan RJ
中科院分区：
文献类型：
--
作者：
Zhang J;Wang Y;Jin JY;Degan S;Hall RP;Boehm RD;Jaipan P;Narayan RJ
伊曲康唑是一种三唑类药物，通常用于治疗指甲感染和其他真菌感染。近期研究表明，伊曲康唑还可通过抑制音猬因子（SHH）信号通路来抑制基底细胞癌（BCC）的生长。在这项研究中，聚乙醇酸微针阵列和不锈钢微针阵列被用于将伊曲康唑经皮递送至在小鼠上再生的人基底细胞癌模型。采用注射成型和拉伸光刻技术制备了具有尖锐针尖的、642μm长的聚乙醇酸微针的1×4阵列。为了进行比较，还获取了连接注射器的85根800μm高的不锈钢微针阵列。将含有失活的中厚人皮片且接种了经转导可表达人SHH蛋白的人角质形成细胞的皮肤移植物缝合到免疫缺陷小鼠的皮肤上。患有这种人基底细胞癌模型的小鼠每天用溶解在60%二甲基亚砜和40%聚乙二醇 - 400溶液中的伊曲康唑治疗2周；通过四个1×4聚乙醇酸微针阵列或不锈钢微针阵列促进伊曲康唑溶液的经皮给药。用聚乙醇酸微针或不锈钢微针处理的表皮组织明显比对照（未处理）的移植物组织薄。这些初步结果表明，微针可用于促进伊曲康唑的经皮递送，以实现基底细胞癌的局部治疗。
Itraconazole is a triazole agent that is routinely used for treatment of nail infections and other fungal infections. Recent studies indicate that itraconazole can also inhibit the growth of basal cell carcinoma (BCC) through suppression of the Sonic Hedgehog (SHH) signaling pathway. In this study, polyglycolic acid microneedle arrays and stainless steel microneedle arrays were used for transdermal delivery of itraconazole to a human BCC model which was regenerated on mice. One-by-four arrays of 642-μm-long polyglycolic acid microneedles with sharp tips were prepared using injection molding and drawing lithography. Arrays of 85 stainless steel 800-μm-tall microneedles attached to syringes were obtained for comparison purposes. Skin grafts containing devitalized split-thickness human dermis that had been seeded with human keratinocytes transduced to express human SHH protein were sutured to the skin of immunodeficient mice. Mice with this human BCC model were treated daily for 2 weeks with itraconazole dissolved in 60% dimethylsulfoxane and 40% polyethylene glycol-400 solution; transdermal administration of the itraconazole solution was facilitated by either four 1 × 4 polyglycolic acid microneedle arrays or stainless steel microneedle arrays. The epidermal tissues treated with polyglycolic acid microneedles or stainless steel microneedles were markedly thinner than that of the control (untreated) graft tissue. These preliminary results indicate that microneedles may be used to facilitate transdermal delivery of itraconazole for localized treatment of BCC.
登录
查看更多内容
影响因子：
10.8
作者：
Park, JH;Allen, MG;Prausnitz, MR
通讯作者：
Prausnitz, MR
影响因子：
82.9
作者：
Fan, HR;Oro, AE;Khavari, PA
通讯作者：
Khavari, PA
影响因子：
50.3
作者：
Kim J;Aftab BT;Tang JY;Kim D;Lee AH;Rezaee M;Kim J;Chen B;King EM;Borodovsky A;Riggins GJ;Epstein EH Jr;Beachy PA;Rudin CM
通讯作者：
Rudin CM
影响因子：
50.3
作者：
Kim J;Tang JY;Gong R;Kim J;Lee JJ;Clemons KV;Chong CR;Chang KS;Fereshteh M;Gardner D;Reya T;Liu JO;Epstein EH;Stevens DA;Beachy PA
通讯作者：
Beachy PA
影响因子：
45.3
作者：
Kim, Daniel J.;Kim, James;Tang, Jean Y.
通讯作者：
Tang, Jean Y.