Microfluidics meet cell biology: bridging the gap by validation and application of microscale techniques for cell biological assays.
Microfluidics meet cell biology: bridging the gap by validation and application of microscale techniques for cell biological assays.
复制标题
DOI：
10.1002/bies.20804
复制
发表时间：
2008-09
期刊：
影响因子：
4
通讯作者：
Beebe, David J.
中科院分区：
文献类型：
--
作者：
Paguirigan, Amy L.;Beebe, David J.
微尺度技术已经应用于生物测定近二十年，但还没有被广泛集成为生物实验室的常用工具。在微观尺度与宏观尺度的几个物理现象之间的显着差异已被利用，以提供各种新类型的测定（如梯度生产或空间细胞图案）。然而，生物学家对这些设备的使用似乎受到生物学验证、易用性以及使用微技术进行测定的可用读数有限等问题的限制。最近已经完成了关键的验证工作，突出了微流体方法当前的挑战，并提出了未来设备可能被改进以更好地与生物测定集成的方法。随着更多的验证和改进的设计，微尺度技术作为一个平台，研究细胞生物学的各个方面，是不可能使用当前的宏观尺度技术的巨大希望。
Microscale techniques have been applied to biological assays for nearly two decades, but haven’t been widely integrated as common tools in biological laboratories. The significant differences between several physical phenomena at the microscale versus the macroscale have been exploited to provide a variety of new types of assays (such as gradient production or spatial cell patterning). However, the use of these devices by biologists seems to be limited by issues regarding biological validation, ease of use, and the limited available readouts for assays done using microtechnology. Critical validation work has been done recently that highlights the current challenges for microfluidic methods and suggest ways in which future devices might be improved to better integrate with biological assays. With more validation and improved designs, microscale techniques hold immense promise as a platform to study aspects of cell biology that are not possible using current macroscale techniques.
登录
查看更多内容
影响因子：
7.4
作者：
Heo, Yun Seok;Cabrera, Lourdes M.;Takayama, Shuichi
通讯作者：
Takayama, Shuichi
影响因子：
1.6
作者：
Fujii, S;Uematsu, M;Sato, K
通讯作者：
Sato, K
影响因子：
0.9
作者：
PURCELL, EM
通讯作者：
PURCELL, EM
影响因子：
7.4
作者：
Koyama, Sachiko;Amarie, Dragos;Jacobson, Stephen C.
通讯作者：
Jacobson, Stephen C.
影响因子：
46.9
作者：
Jeon, NL;Baskaran, H;Toner, M
通讯作者：
Toner, M