New small nuclear RNA gene-like transcriptional units as sources of regulatory transcripts.
New small nuclear RNA gene-like transcriptional units as sources of regulatory transcripts.
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新的小核RNA基因样转录单元作为调节转录的来源。
DOI：
10.1371/journal.pgen.0030001
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发表时间：
2007-02-02
期刊：
影响因子：
4.5
通讯作者：
Cancedda R
中科院分区：
文献类型：
--
作者：
Pagano A;Castelnuovo M;Tortelli F;Ferrari R;Dieci G;Cancedda R
通过计算机搜索典型的小核RNA基因的上游启动子元件（远端序列元件和近端序列元件），我们已经在人类基因组中确定了一些以前未被识别的假定转录单位，其预测产物是与蛋白质编码基因同源的新型非编码RNA。通过阐明其中之一的功能，我们提供的证据存在的正义/反义为基础的基因调控网络的聚合酶III转录组的一部分可以控制其聚合酶II对应。在人类基因组的序列被确定后，人们立即认识到，在生理和病理条件下，细胞中发生的基因表达的大部分调节是由不编码蛋白质的RNA分子（基因组的“非编码部分”）进行的。在这里，我们专注于RNA聚合酶III转录的小RNA分子，并确定了一套新的约30个非编码（NC）RNA基因。我们建议，这些RNA转录发挥关键作用，在调节特定的蛋白质编码基因的表达转录的RNA聚合酶II，从而构成了一个前所未有的例子cogene/基因对。此外，我们提供的证据表明，RNA聚合酶III，除了众所周知的小RNA（如5S rRNA和tRNA）的组成性合成的任务，也发挥了关键作用，在该地区的基因表达控制。对一种称为21 A的新型ncRNA基因的功能的详细研究表明，它的转录本在控制某些肿瘤细胞的增殖中起作用。上述发现显着扩大了我们对ncRNA宇宙的理解，并为进一步研究开辟了道路，旨在阐明涉及这类新型调控RNA的分子途径。
By means of a computer search for upstream promoter elements (distal sequence element and proximal sequence element) typical of small nuclear RNA genes, we have identified in the human genome a number of previously unrecognized, putative transcription units whose predicted products are novel noncoding RNAs with homology to protein-coding genes. By elucidating the function of one of them, we provide evidence for the existence of a sense/antisense-based gene-regulation network where part of the polymerase III transcriptome could control its polymerase II counterpart. After the sequence of the human genome was determined, it was immediately recognized that a large part of the regulation of the gene expression occurring in the cells under physiological, as well as under pathological conditions, is carried out by RNA molecules that do not code for proteins (the “noncoding portion” of the genome). Here, we focus on small RNA molecules transcribed by the RNA polymerase III and identify a novel set of approximately 30 noncoding (nc) RNA genes. We propose that these RNA transcripts play a key role in regulating the expression of specific protein-coding genes transcribed by the RNA polymerase II, thus constituting an unprecedented example of cogene/gene pairs. Furthermore, we provide evidence that the RNA polymerase III, in addition to the well-known task in the constitutive synthesis of small RNAs (such as 5S rRNA and tRNAs), also plays a key role in the area of gene-expression control. A detailed investigation of the function of one of the novel ncRNA genes, called 21A, revealed that its transcript plays a role in the control of the proliferation of some tumor cells. The above findings significantly expand our understanding of the ncRNA universe and open the way to further studies aimed at the elucidation of the molecular pathways involving this novel class of regulatory RNAs.
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影响因子：
56.9
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Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者：
Hayashizaki, Y
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