Assessment of dual life stage antiplasmodial activity of british seaweeds.
Assessment of dual life stage antiplasmodial activity of british seaweeds.
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DOI：
10.3390/md11104019
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发表时间：
2013-10-22
期刊：
影响因子：
5.4
通讯作者：
Tasdemir D
中科院分区：
文献类型：
--
作者：
Spavieri J;Allmendinger A;Kaiser M;Itoe MA;Blunden G;Mota MM;Tasdemir D
陆生植物已被证明是临床有效的抗疟药物的多产生产者，但海藻的抗疟潜力却很少被探索。本研究的主要目的是评估从英国南部海岸采集的23种海藻提取物对血期（BS）和肝期（LS）疟原虫的体外化疗和预防作用。大部分提取物（14份）对恶性疟原虫有抑制作用，其中褐藻柽柳囊藻（Cystoseira tamariscifolia）、黑藻（C. baccata）和绿藻Ulva lactuca的抑菌活性最高（ic50值约为3 μg/mL）。提取物具有较高的选择性指数（bbb10）。8种海藻提取物均能抑制伯氏螺旋体LS寄生虫的生长，但对人肝癌（Huh7）细胞的活性无明显影响，其中乳藻和红海藻（Ceramium virgatum和Halopitys incurvus）的IC50值最高（IC50值为14.9 ~ 28.8 μg/mL）。LS活性提取物可抑制疟疾ii型脂肪酸生物合成（FAS-II）途径的一个或多个关键酶，这是一种针对LS的药物靶点。除红藻外，所有活性提取物对两种疟疾细胞内寄生虫均具有双重活性。这是首次报道海藻的LS抗疟原虫活性和双阶段抑制潜能。
Terrestrial plants have proven to be a prolific producer of clinically effective antimalarial drugs, but the antimalarial potential of seaweeds has been little explored. The main aim of this study was to assess the in vitro chemotherapeutical and prophylactic potential of the extracts of twenty-three seaweeds collected from the south coast of England against blood stage (BS) and liver stage (LS) Plasmodium parasites. The majority (14) of the extracts were active against BS of P. falciparum, with brown seaweeds Cystoseira tamariscifolia, C. baccata and the green seaweed Ulva lactuca being the most active (IC50s around 3 μg/mL). The extracts generally had high selectivity indices (>10). Eight seaweed extracts inhibited the growth of LS parasites of P. berghei without any obvious effect on the viability of the human hepatoma (Huh7) cells, and the highest potential was exerted by U. lactuca and red seaweeds Ceramium virgatum and Halopitys incurvus (IC50 values 14.9 to 28.8 μg/mL). The LS-active extracts inhibited one or more key enzymes of the malarial type-II fatty acid biosynthesis (FAS-II) pathway, a drug target specific for LS. Except for the red seaweed Halopitys incurvus, all LS-active extracts showed dual activity versus both malarial intracellular stage parasites. This is the first report of LS antiplasmodial activity and dual stage inhibitory potential of seaweeds.
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影响因子：
3.8
作者：
Afolayan, Anthonia F.;Mann, Maryssa G. A.;Beukes, Denzil R.
通讯作者：
Beukes, Denzil R.
影响因子：
2
作者：
Ravikumar, Sundaram;Inbaneson, Samuel Jacob;Venkatesan, Malaiyandi
通讯作者：
Venkatesan, Malaiyandi
影响因子：
7.2
作者：
Spavieri, Jasmine;Kaiser, Marcel;Tasdemir, Deniz
通讯作者：
Tasdemir, Deniz
影响因子：
3.7
作者：
Ploemen IH;Prudêncio M;Douradinha BG;Ramesar J;Fonager J;van Gemert GJ;Luty AJ;Hermsen CC;Sauerwein RW;Baptista FG;Mota MM;Waters AP;Que I;Lowik CW;Khan SM;Janse CJ;Franke-Fayard BM
通讯作者：
Franke-Fayard BM
DOI：
10.1073/pnas.1306097110
发表时间：
2013-07-23
影响因子：
11.1
作者：
Hanson, Kirsten K.;Ressurreicao, Ana S.;Mota, Maria M.
通讯作者：
Mota, Maria M.