Effects of dietary energy levels on rumen bacterial community composition in Holstein heifers under the same forage to concentrate ratio condition.
Effects of dietary energy levels on rumen bacterial community composition in Holstein heifers under the same forage to concentrate ratio condition.
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DOI：
10.1186/s12866-018-1213-9
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发表时间：
2018-07-11
期刊：
影响因子：
4.2
通讯作者：
Tu Y
中科院分区：
文献类型：
--
作者：
Bi Y;Zeng S;Zhang R;Diao Q;Tu Y
瘤胃细菌群落在饲料降解和生产力方面发挥着关键作用。不同草精料比例对瘤胃微生物种群结构的影响已得到广泛研究；然而，对于饲喂不同能量水平日粮且草料与精料比例相同的小母牛瘤胃细菌群落组成变化的研究非常有限。本研究的目的是探讨不同日粮能量水平、相同草精料比例对小母牛瘤胃细菌群落组成的影响。此外，我们还确定了瘤胃细菌与瘤胃发酵参数之间的关系。 16S rRNA基因测序表明，在50:50的草精比下，日粮能量水平8%的差异对小母牛主要门和大部分主要属的α多样性和相对丰度没有显着影响。在所有治疗组中，厚壁菌门、拟杆菌门和变形菌门是主要门。瘤胃细菌属水平相对丰度与发酵参数的Spearman相关分析表明，普雷沃氏菌属和BF311属的相对丰度与氨氮和丁酸盐浓度呈正相关，这两个属分别与丙酸盐和异戊酸盐浓度呈负相关，双歧杆菌属与发酵参数呈正相关。 丁酸盐浓度与丙酸盐和异戊酸盐浓度呈负相关。总挥发性脂肪酸浓度与BF311丰度呈正相关，与毛球菌属和Facklamia丰度呈负相关。在相同草精比50:50的条件下，日粮能量水平差异8%对小母牛瘤胃细菌群落组成影响不大。瘤胃细菌的某些属与挥发性脂肪酸和氨氮浓度之间的相关性可能表明瘤胃发酵参数受到瘤胃细菌群落组成的强烈影响。
The rumen bacterial community plays a critical role in feeds degradation and productivity. The effects of different forage to concentrate ratios on the ruminal microbial population structure have been studied extensively; however, research into changes in the ruminal bacterial community composition in heifers fed different energy level diets, with the same forage to concentrate ratio, has been very limited. The purpose of this study was to investigate the effects of different dietary energy levels, with the same forage to concentrate ratio, on ruminal bacterial community composition of heifers. Furthermore, we also determine the relationship between rumen bacteria and ruminal fermentation parameters. The 16S rRNA gene sequencing showed that, under the same forage to concentrate ratio of 50:50, an 8% difference in dietary energy level had no significant impact on the alpha diversity and the relative abundance of the major phyla and most of the major genera in heifers. In all the treatments groups, Firmicutes, Bacteroidetes, and Proteobacteria were the dominant phyla. Spearman correlation analysis between the relative abundances of the rumen bacteria at the genus level and the fermentation parameters showed that the relative abundances of Prevotella and BF311 were positively correlated with the ammonia nitrogen and butyrate concentrations, and these two genera were negatively correlated with the propionate and isovalerate concentrations, respectively, and the genus Bifidobacterium was positively correlated with the butyrate concentration and was negatively correlated with propionate and isovalerate concentration. The total volatile fatty acid concentration was positively correlated with BF311 abundances, and was negatively correlated with Trichococcus and Facklamia abundances. Under the same forage to concentrate ratio condition of 50:50, an 8% difference in dietary energy levels had little impact on rumen bacterial community composition in heifers. The correlations between some genera of ruminal bacteria and the concentrations of volatile fatty acids and ammonia nitrogen might be indicative that the ruminal fermentation parameters are strongly influenced by the rumen bacterial community composition.
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影响因子：
5.4
作者：
Dicks, L. M. T.;Botes, M.
通讯作者：
Botes, M.
影响因子：
4.4
作者：
Fernando, S. C.;Purvis, H. T., II;DeSilva, U.
通讯作者：
DeSilva, U.
DOI：
10.1111/j.1365-2672.1995.tb03119.x
发表时间：
1995-07-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
ATTWOOD, GT;REILLY, K
通讯作者：
REILLY, K
影响因子：
4.4
作者：
Amato, Pierre;Christner, Brent C.
通讯作者：
Christner, Brent C.
影响因子：
2.1
作者：
Guan, Le Luo;Nkrumah, Joshua D.;Moore, Stephen S.
通讯作者：
Moore, Stephen S.