Hand-held high-resolution fluorescence imaging system for fluorescence-guided surgery of patient and cell-line pancreatic tumors growing orthotopically in nude mice.
Hand-held high-resolution fluorescence imaging system for fluorescence-guided surgery of patient and cell-line pancreatic tumors growing orthotopically in nude mice.
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DOI：
10.1016/j.jss.2013.11.1083
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发表时间：
2014-04
影响因子：
2.2
通讯作者：
Hoffman, Robert M.
中科院分区：
文献类型：
--
作者：
Hiroshima, Yukihiko;Maawy, Ali;Sato, Sho;Murakami, Takashi;Uehara, Fuminari;Miwa, Shinji;Yano, Shuya;Momiyama, Masashi;Chishima, Takashi;Tanaka, Kuniya;Bouvet, Michael;Endo, Itaru;Hoffman, Robert M.
关键词：
在这项研究中，我们调查了一个便携式的手大小的成像系统相比，一个大腔荧光成像系统或长工作距离荧光显微镜的荧光引导手术（FGS）在小鼠中的优势。用于FGS的人胰腺癌的小鼠模型包括（1）表达MiaPaCa-2的绿色荧光蛋白（GFP），（2）用与Alexa 488缀合的抗CEA抗体标记的BxPC 3，（3）用与Alexa 488缀合的抗CA 19 -9抗体标记的患者来源的原位异种移植物（PDOX）™。每个装置都可以清楚地检测到初级MiaPaCa-2-GFP。肿瘤和FGS后的任何残留肿瘤。在用Alexa 488缀合的抗CEA标记的BxPC 3模型中，每个装置都可以检测原发性肿瘤，但是MVX 10不能清楚地检测FGS后剩余的残留肿瘤，而其他装置可以。在用与抗CA 19 -9缀合的Alexa 488标记的PDOX™模型中，仅便携式手持装置可以将残余肿瘤与背景区分开，并且在荧光导航下实现了残余肿瘤的完全切除。本报告中描述的结果表明，手持式移动的成像系统由于其方便的尺寸和高灵敏度而能够应用于FGS的临床，并有助于FGS的广泛应用。
In this study, we investigated the advantages for fluorescence-guided surgery (FGS) in mice of a portable hand-sized imaging system compared to a large chamber fluorescing imaging system or a long-working-distance fluorescence microscope. Mouse models of human pancreatic cancer for FGS included (1) MiaPaCa-2-expressing green fluorescent protein (GFP), (2) BxPC3 labeled with anti-CEA antibody conjugated with Alexa 488, (3) patient-derived orhotopic xenograft (PDOX)™ labeled with anti-CA19-9 antibody conjugated with Alexa 488. Each device could clearly detect the primary MiaPaCa-2-GFP. tumor and any residual tumor after FGS. In the BxPC3 model labeled with Alexa 488-conjugated anti-CEA, each device could detect the primary tumor, but the MVX10 could not clearly detect the residual tumor remaining after FGS while the other devices could. In the PDOX™ model labeled with Alexa 488 conjugated with anti CA19-9, only the portable hand-held device could distinguish the residual tumor from the background, and complete resection of the residual tumor was achieved under fluorescence navigation. The results described in the present report suggest the hand-held mobile imaging system can be able to be applied to the clinic for FGS due to its convenient size and high sensitivity and help make FGS widely-used.
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DOI：
10.1016/j.jamcollsurg.2012.02.021
发表时间：
2012-07
影响因子：
5.2
作者：
Metildi, Cristina A.;Kaushal, Sharmeela;Hardamon, Chanae R.;Snyder, Cynthia S.;Pu, Minya;Messer, Karen S.;Talamini, Mark A.;Hoffman, Robert M.;Bouvet, Michael
通讯作者：
Bouvet, Michael
影响因子：
--
作者：
Tran Cao HS;Kaushal S;Metildi CA;Menen RS;Lee C;Snyder CS;Messer K;Pu M;Luiken GA;Talamini MA;Hoffman RM;Bouvet M
通讯作者：
Bouvet M
DOI：
10.1073/pnas.89.12.5645
发表时间：
1992-06-15
影响因子：
11.1
作者：
FU, XY;GUADAGNI, F;HOFFMAN, RM
通讯作者：
HOFFMAN, RM
影响因子：
5.7
作者：
Cao, Hop S. Tran;Bouvet, Michael;Katz, Matthew H. G.
通讯作者：
Katz, Matthew H. G.
影响因子：
3.2
作者：
Kaushal, Sharmeela;McElroy, Michele K.;Luiken, George A.;Talamini, Mark A.;Moossa, A. R.;Hoffman, Robert M.;Bouvet, Michael
通讯作者：
Bouvet, Michael