A comprehensive microRNA expression profile of the backfat tissue from castrated and intact full-sib pair male pigs.
A comprehensive microRNA expression profile of the backfat tissue from castrated and intact full-sib pair male pigs.
复制标题
去势和完整同胞对雄性猪背膘组织的全面 microRNA 表达谱
DOI：
10.1186/1471-2164-15-47
复制
发表时间：
2014-01-20
期刊：
影响因子：
4.4
通讯作者：
Fang MY
中科院分区：
文献类型：
--
作者：
Bai Y;Huang JM;Liu G;Zhang JB;Wang JY;Liu CK;Fang MY
背景众所周知，去势对脂肪组织的积累有显著影响。已知microRNA（miRNAs）参与脂肪沉积并受雄激素诱导的雄激素受体（AR）调节。然而，对miRNAs与去势后脂肪沉积之间的关系了解甚少。在这项研究中，高通量SOLiD测序方法用于鉴定和表征来自完整和阉割的全同胞雄性23周龄猪的背膘中的miRNA表达。比较了去势和完整雄性猪脂肪形成和脂肪沉积的模式。ResultsA共鉴定出366个独特的miRNA基因，包括174个已知的前体miRNA和192个新的前体miRNA。167个pre-miRNA对于去势（F3）和完整（F4）雄性猪小RNA文库是共同的。在F3和F4文库中，新的pre-miRNA分别编码153个miRNA/miRNA*s和141个miRNA/miRNA*s。177个miRNAs，包括45个上调和132个下调，在去势和完整的雄性猪之间具有超过2倍的差异表达（p值< 0.001）。基于两个文库之间的表达丰度和差异，进一步选择了35个miRNA，以预测它们在KEGG通路中的靶标。KEGG通路分析表明，去势公猪与正常公猪差异表达的miRNAs参与了细胞增殖、凋亡、分化、迁移、脂肪组织发育等重要的生物学过程。通过茎环逆转录定量聚合酶链反应验证了8个任意选择的miRNA的表达模式。这些数据证实了SOLiD测序观察到的表达趋势。在本研究中还研究了miRNA isomiR和mirtron。Mirtron是最近描述的一类miRNA，其依赖于剪接而不是通过微处理器复合物加工以产生RNAi途径。本研究还对调节脂肪沉积的重要miRNAs的功能进行了研究。结论本研究扩大了猪脂肪沉积相关miRNAs的数量。结果还表明，去势可以显著影响脂肪相关miRNA的表达模式。差异表达的miRNAs可能在去势后脂肪沉积中起重要作用。
BackgroundIt is widely known that castration has a significant effect on the accumulation of adipose tissue. microRNAs (miRNAs) are known to be involved in fat deposition and to be regulated by the androgen-induced androgen receptor (AR). However, there is little understanding of the relationship between miRNAs and fat deposition after castration. In this study, the high-throughput SOLiD sequencing approach was used to identify and characterize miRNA expression in backfat from intact and castrated full-sib male 23-week-old pigs. The patterns of adipogenesis and fat deposition were compared between castrated and intact male pigs.ResultsA total of 366 unique miRNA genes were identified, comprising 174 known pre-miRNAs and 192 novel pre-miRNAs. One hundred and sixty-seven pre-miRNAs were common to both castrated (F3) and intact (F4) male pig small RNA libraries. The novel pre-miRNAs encoded 153 miRNAs/miRNA*s and 141 miRNAs/miRNA*s in the F3 and F4 libraries, respectively. One hundred and seventy-seven miRNAs, including 45 up- and 132 down-regulated, had more than 2-fold differential expression between the castrated and intact male pigs (p-value < 0.001). Thirty-five miRNAs were further selected, based on the expression abundance and differentiation between the two libraries, to predict their targets in KEGG pathways. KEGG pathway analyses suggested that miRNAs differentially expressed between the castrated and intact male pigs are involved in proliferation, apoptosis, differentiation, migration, adipose tissue development and other important biological processes. The expression patterns of eight arbitrarily selected miRNAs were validated by stem-loop reverse-transcription quantitative polymerase chain reaction. These data confirmed the expression tendency observed with SOLiD sequencing. miRNA isomiRs and mirtrons were also investigated in this study. Mirtrons are a recently described category of miRNA relying on splicing rather than processing by the microprocessor complex to generate the RNAi pathway. The functions of miRNAs important for regulating fat deposition were also investigated in this study.ConclusionsThis study expands the number of fat-deposition-related miRNAs in pig. The results also indicate that castration can significantly affect the expression patterns of fat-related miRNAs. The differentially expressed miRNAs may play important roles in fat deposition after castration.
登录
查看更多内容
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
12.3
作者：
Hackl H;Burkard TR;Sturn A;Rubio R;Schleiffer A;Tian S;Quackenbush J;Eisenhaber F;Trajanoski Z
通讯作者：
Trajanoski Z
影响因子：
9.2
作者：
Huang J;Ju Z;Li Q;Hou Q;Wang C;Li J;Li R;Wang L;Sun T;Hang S;Gao Y;Hou M;Zhong J
通讯作者：
Zhong J
影响因子：
3.7
作者：
Chartoumpekis DV;Zaravinos A;Ziros PG;Iskrenova RP;Psyrogiannis AI;Kyriazopoulou VE;Habeos IG
通讯作者：
Habeos IG
影响因子：
4.1
作者：
Li, Hui-Xia;Luo, Xiao;Yang, Gong-She
通讯作者：
Yang, Gong-She