Single cell RNA sequencing approaches to cardiac development and congenital heart disease.
Single cell RNA sequencing approaches to cardiac development and congenital heart disease.
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单细胞RNA测序方法用于心脏发育和先天性心脏病。
DOI：
10.1016/j.semcdb.2021.04.023
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发表时间：
2021-10
影响因子：
7.3
通讯作者：
Wu SM
中科院分区：
文献类型：
--
作者：
Samad T;Wu SM
单细胞RNA测序技术的发展加速了科学家了解心血管系统健康和疾病状态的能力。每年约有40，000名新生儿发生先天性心脏病，每4名儿童中就有1名出生时患有严重的先天性心脏病，需要手术干预和终身监测。了解正常心脏如何发育以及每个细胞如何对正常和病理解剖结构做出贡献是儿科心血管研究的重要目标。单细胞测序提供了工具，以提高发现罕见的细胞类型和新的基因参与正常心脏发育的能力。心脏组织内单个细胞基因表达的知识有助于理解每种细胞类型如何对心脏的解剖结构做出贡献。在这篇综述中，我们总结了如何单细胞RNA测序已被用来了解心脏发育过程和先天性心脏病。我们讨论了全细胞与单核RNA测序的优点和缺点，并描述了从单细胞数据分析相互作用组、转录组和分化轨迹的方法。我们总结了目前可用的单细胞RNA测序技术和执行单细胞分析的技术方面，以及如何克服常见的障碍。我们还回顾了最近发表的人类和小鼠胎心图谱的数据，以及由于这些单细胞工具的应用而在该领域取得的进展。最后，我们强调了单细胞技术通过利用全基因组关联研究的结果来揭示疾病发病机制的新机制的潜力。
The development of single cell RNA sequencing technologies has accelerated the ability of scientists to understand healthy and disease states of the cardiovascular system. Congenital heart defects occur in approximately 40,000 births each year and 1 out of 4 children are born with critical congenital heart disease requiring surgical interventions and a lifetime of monitoring. An understanding of how the normal heart develops and how each cell contributes to normal and pathological anatomy is an important goal in pediatric cardiovascular research. Single cell sequencing has provided the tools to increase the ability to discover rare cell types and novel genes involved in normal cardiac development. Knowledge of gene expression of single cells within cardiac tissue has contributed to the understanding of how each cell type contributes to the anatomic structures of the heart. In this review, we summarize how single cell RNA sequencing has been utilized to understand cardiac developmental processes and congenital heart disease. We discuss the advantages and disadvantages of whole cell versus single nuclei RNA sequencing and describe the approaches to analyze the interactomes, transcriptomes, and differentiation trajectory from single cell data. We summarize the currently available single cell RNA sequencing technologies and technical aspects of performing single cell analysis and how to overcome common obstacles. We also review data from the recently published human and mouse fetal heart atlases and advancements that have occurred within the field due to the application of these single cell tools. Finally we highlight the potential for single cell technologies to uncover novel mechanisms of disease pathogenesis by leveraging findings from genome wide association studies.
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