Light-induced effects of a fluorescent voltage-sensitive dye on neuronal activity in the crab stomatogastric ganglion
Light-induced effects of a fluorescent voltage-sensitive dye on neuronal activity in the crab stomatogastric ganglion
复制标题
荧光电压敏感染料对蟹口胃神经节神经元活动的光诱导作用
DOI：
10.1016/j.jneumeth.2010.03.003
复制
发表时间：
2010
影响因子：
3
通讯作者：
Péter András
中科院分区：
文献类型：
--
作者：
W. Stein;Péter András
光学成像是研究神经活动的前沿方法之一，了解染料分子的工作机制及其可能引起的副作用范围非常重要。特别是，揭示潜在的毒性作用和损害所研究的神经系统功能的影响是非常重要的。在这里，我们研究了在常用的di-4-ANEPPS电压敏感染料存在的情况下，照明对螃蟹口胃神经系统（运动模式产生的模型系统）幽门中枢模式发生器产生的节律性运动模式的影响。我们报告说，染料允许长时间录音，几乎没有漂白，没有明显损害幽门节奏。然而，令人兴奋的光照诱导幽门运动神经元的相位关系发生了暂时的可逆变化，并伴随着节奏的加快。这种效应与di-4-ANEPPS的激发波长有关，只有在神经堆和细胞体被照射时才会产生。因此，di-4-ANEPPS作为一个光开关，导致运动神经元的相位关系发生快速可逆的变化，但不会对神经元功能造成永久性损害。因此，它也可以作为研究有节奏运动模式中相位关系维持的一种手段。
Optical imaging being one of the cutting-edge methods for the investigation of neural activity, it is very important to understand the mechanisms of how dye molecules work and the range of side effects that they may induce. In particular, it is very important to reveal potential toxic effects and effects impairing the functioning of the investigated neural system. Here, we investigate the effects of illumination in the presence of the commonly used di-4-ANEPPS voltage-sensitive dye on the rhythmic motor pattern generated by the pyloric central pattern generator in the crab stomatogastric nervous system, a model system for motor pattern generation. We report that the dye allows long recording sessions with little bleaching and no obvious damage to the pyloric rhythm. Yet, exciting illumination induced a temporary and reversible change in the phase relationship of the pyloric motor neurons and a concomitant speed-up of the rhythm. The effect was specific to the excitation wavelength of di-4-ANEPPS and only obtained when the neuropile and cell bodies were illuminated. Thus, di-4-ANEPPS acts as a photo-switch that causes a quick and reversible change in the phase relationship of the motor neurons, but no permanent impairment of neuronal function. It may thus also be used as a means to study the maintenance of phase relationships in rhythmic motor patterns.
蟹口胃神经节振荡神经元的空间和时间分辨钙浓度变化。
DOI：
10.1073/pnas.86.5.1679
发表时间：
1989
影响因子：
11.1
作者：
Ross,WN;Graubard,K
通讯作者：
Graubard,K
影响因子：
5.3
作者：
Bucher, D;Prinz, AA;Marder, E
通讯作者：
Marder, E
影响因子：
2.9
作者：
FLUHLER, E;BURNHAM, VG;LOEW, LM
通讯作者：
LOEW, LM