Enhanced chondrogenesis in co-cultures with articular chondrocytes and mesenchymal stem cells.
Enhanced chondrogenesis in co-cultures with articular chondrocytes and mesenchymal stem cells.
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与关节软骨细胞和间充质干细胞共培养的软骨发生增强。
DOI：
10.1016/j.biomaterials.2012.05.042
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发表时间：
2012-09
期刊：
影响因子：
14
通讯作者：
Mikos, Antonios G.
中科院分区：
文献类型：
--
作者：
Meretoja, Ville V.;Dahlin, Rebecca L.;Kasper, F. Kurtis;Mikos, Antonios G.
在这项工作中，关节软骨细胞（AC）和间充质干细胞（MSC）以1：1和1：3的细胞比例共培养，以评估是否可以用MSC替代大多数原代AC，而不会对体外软骨形成产生不利影响。我们进一步使用异种培养模型来研究这种共培养是否可以导致传代AC的再分化。将细胞在多孔支架中培养四周，并测量其细胞构成、软骨样基质形成和软骨形成基因表达水平（胶原蛋白I和II、聚集蛋白聚糖）。与培养扩增的软骨细胞或从相同动物收获的MSC相比，具有原代牛AC的构建体的最终DNA和糖胺聚糖含量分别高约1.6倍和5.5倍。在共培养物中也观察到同样稳健的软骨形成，即使最初用MSC替换高达75%的原代AC。此外，物种特异性RT-PCR分析表明，在牛-兔共培养物中逐渐丧失MSC。最后，使用原代和培养扩增的AC的共培养导致类似的结果。我们得出结论，软骨工程最有前途的细胞来源是共培养物，因为MSC的营养作用可以极大地增加AC的软骨形成潜力，从而减少初级软骨细胞收获和扩增的问题。
In this work, articular chondrocytes (ACs) and mesenchymal stem cells (MSCs) with 1:1 and 1:3 cell ratios were co-cultured in order to evaluate if a majority of primary ACs can be replaced with MSCs without detrimental effects on in vitro chondrogenesis. We further used a xenogeneic culture model to study if such co-cultures can result in redifferentiation of passaged ACs. Cells were cultured in porous scaffolds for four weeks and their cellularity, cartilage-like matrix formation and chondrogenic gene expression levels (collagen I and II, aggrecan) were measured. Constructs with primary bovine ACs had ~1.6 and 5.5 times higher final DNA and glycosaminoglycan contents, respectively, in comparison to those with culture expanded chondrocytes or MSCs harvested from the same animals. Equally robust chondrogenesis was also observed in co-cultures, even when up to 75% of primary ACs were initially replaced with MSCs. Furthermore, species-specific RT-PCR analysis indicated a gradual loss of MSCs in bovine-rabbit co-cultures. Finally, co-cultures using primary and culture expanded ACs resulted in similar outcomes. We conclude that the most promising cell source for cartilage engineering was the co-cultures, as the trophic effect of MSCs may highly increase the chondrogenic potential of ACs thus diminishing the problems with primary chondrocyte harvest and expansion.
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影响因子：
5.6
作者：
Dickhut, Andrea;Pelttari, Karoliina;Richter, Wiltrud
通讯作者：
Richter, Wiltrud
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
体内骨化的体内骨骼通过体外软骨内骨化的骨骼骨化，对成年人和大鼠间充质干细胞的体内造成启动。
影响因子：
2.3
作者：
Farrell E;Both SK;Odörfer KI;Koevoet W;Kops N;O'Brien FJ;Baatenburg de Jong RJ;Verhaar JA;Cuijpers V;Jansen J;Erben RG;van Osch GJ
通讯作者：
van Osch GJ
影响因子：
14
作者：
Liao, Jiehong;Guo, Xuan;Grande-Allen, K. Jane;Kasper, F. Kurtis;Mikos, Antonios G.
通讯作者：
Mikos, Antonios G.
影响因子：
4.1
作者：
Ahmed, Nazish;Gan, Lu;Kandel, Rita A.
通讯作者：
Kandel, Rita A.