Bioactive polymer/extracellular matrix scaffolds fabricated with a flow perfusion bioreactor for cartilage tissue engineering.
Bioactive polymer/extracellular matrix scaffolds fabricated with a flow perfusion bioreactor for cartilage tissue engineering.
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DOI：
10.1016/j.biomaterials.2010.07.110
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发表时间：
2010-12
期刊：
影响因子：
14
通讯作者：
Mikos, Antonios G.
中科院分区：
文献类型：
--
作者：
Liao, Jiehong;Guo, Xuan;Grande-Allen, K. Jane;Kasper, F. Kurtis;Mikos, Antonios G.
关键词：
在本研究中，通过在流动灌流生物反应器中动态培养软骨细胞，然后脱细胞构建的方法，制备了包覆有软骨细胞外基质的电纺聚(ε-己内酯)微纤维支架。脱细胞过程得到了含有糖胺聚糖和胶原的无细胞PCL/ECM复合支架。在体外无血清培养条件下，观察复合支架对骨髓间充质干细胞向软骨细胞分化的支持作用，并在无血清条件下分别加入转化生长因子-β-1和转化生长因子-β-1。PCL/ECM复合支架支持转化生长因子-β-1诱导的软骨细胞分化，聚集素和II型胶原基因表达上调，分别为(11.6±3.8)和(668.4±317.7)倍。单独软骨基质的存在降低了I型胶原基因的表达，达到了转化生长因子-β1治疗观察到的水平。软骨基质进一步增强了生长因子处理对MSC软骨形成的影响，在PCL/ECM复合支架上培养的细胞具有较高的糖胺聚糖合成活性。因此，在电纺超细纤维支架上进行软骨细胞的流动灌流培养是一种很有前途的方法，可以构建包含天然成分和合成成分的聚合物/细胞外基质复合支架，为软骨组织工程应用提供生物信号。
In this study, electrospun poly(ε-caprolactone) (PCL) microfiber scaffolds, coated with cartilaginous extracellular matrix (ECM), were fabricated by first culturing chondrocytes under dynamic conditions in a flow perfusion bioreactor and then decellularizing the cellular constructs. The decellularization procedure yielded acellular PCL/ECM composite scaffolds containing glycosaminoglycan and collagen. PCL/ECM composite scaffolds were evaluated for their ability to support the chondrogenic differentiation of mesenchymal stem cells (MSCs) in vitro using serum-free medium with or without the addition of transforming growth factor-β1 (TGF-β1). PCL/ECM composite scaffolds supported chondrogenic differentiation induced by TGF-β1 exposure, as evidenced in the up-regulation of aggrecan (11.6 ± 3.8 fold) and collagen type II (668.4 ± 317.7 fold) gene expression. The presence of cartilaginous matrix alone reduced collagen type I gene expression to levels observed with TGF-β1 treatment. Cartilaginous matrix further enhanced the effects of growth factor treatment on MSC chondrogenesis as evidenced in the higher glycosaminoglycan synthetic activity for cells cultured on PCL/ECM composite scaffolds. Therefore, flow perfusion culture of chondrocytes on electrospun microfiber scaffolds is a promising method to fabricate polymer/extracellular matrix composite scaffolds that incorporate both natural and synthetic components to provide biological signals for cartilage tissue engineering applications.
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影响因子：
9.7
作者：
Liao, Jiehong;Guo, Xuan;Nelson, Dan;Kasper, F. Kurtis;Mikos, Antonios G.
通讯作者：
Mikos, Antonios G.
影响因子：
--
作者：
Farrell, E;O'Brien, FJ;Campbell, VA
通讯作者：
Campbell, VA
影响因子：
--
作者：
Gomes, ME;Bossano, CM;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
14
作者：
Li, WJ;Tuli, R;Tuan, RS
通讯作者：
Tuan, RS
影响因子：
14
作者：
Hu, JC;Athanasiou, KA
通讯作者：
Athanasiou, KA