Dynamic changes in short- and long-term bacterial composition following fecal microbiota transplantation for recurrent Clostridium difficile infection.
Dynamic changes in short- and long-term bacterial composition following fecal microbiota transplantation for recurrent Clostridium difficile infection.
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DOI：
10.1186/s40168-015-0070-0
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发表时间：
2015
期刊：
影响因子：
15.5
通讯作者：
Sadowsky MJ
中科院分区：
文献类型：
--
作者：
Weingarden A;González A;Vázquez-Baeza Y;Weiss S;Humphry G;Berg-Lyons D;Knights D;Unno T;Bobr A;Kang J;Khoruts A;Knight R;Sadowsky MJ
粪便微生物群移植（FMT）是一种有效的治疗复发性艰难梭菌感染（CDI）的方法，通常无法使用标准抗生素治疗。然而，尽管其最近被广泛使用，但对FMT后粪便微生物群的稳定性知之甚少。在这里，我们报告了短期和长期变化，并提供了多次复发的CDI患者粪便微生物群组成的动态可视化，这些患者对抗生素治疗无效，并使用FMT治疗。在FMT前和FMT后151天内从4名患者采集粪便样本，每天采集直至FMT后28天，每周采集直至FMT后84天。使用高通量16 S rRNA基因序列分析来表征粪便细菌的组成，与人类微生物组计划（HMP）数据库中的身体部位的微生物群进行比较，并以电影样的动力学格式可视化。FMT导致细菌粪便样品组成从明显的生态失调状态快速正常化为正常粪便微生物群的代表。虽然微生物组在FMT后1天看起来与供体植入材料最相似，但在随后的时间点，该组合物的差异很小。供体微生物群组成也随时间变化。然而，FMT后和供体样品都保持在HMP表征为健康的较大粪便微生物群云内。动态行为是正常粪便微生物群的固有特性，在比较正常个体和疾病个体之间的微生物群落时应考虑到动态行为。这也表明，需要更频繁地进行样本分析，以便适当评估FMT程序的成功。本文的在线版本（doi：10.1186/s40168-015-0070-0）包含补充材料，可供授权用户使用。
Fecal microbiota transplantation (FMT) is an effective treatment for recurrent Clostridium difficile infection (CDI) that often fails standard antibiotic therapy. Despite its widespread recent use, however, little is known about the stability of the fecal microbiota following FMT. Here we report on short- and long-term changes and provide kinetic visualization of fecal microbiota composition in patients with multiply recurrent CDI that were refractory to antibiotic therapy and treated using FMT. Fecal samples were collected from four patients before and up to 151 days after FMT, with daily collections until 28 days and weekly collections until 84 days post-FMT. The composition of fecal bacteria was characterized using high throughput 16S rRNA gene sequence analysis, compared to microbiota across body sites in the Human Microbiome Project (HMP) database, and visualized in a movie-like, kinetic format. FMT resulted in rapid normalization of bacterial fecal sample composition from a markedly dysbiotic state to one representative of normal fecal microbiota. While the microbiome appeared most similar to the donor implant material 1 day post-FMT, the composition diverged variably at later time points. The donor microbiota composition also varied over time. However, both post-FMT and donor samples remained within the larger cloud of fecal microbiota characterized as healthy by the HMP. Dynamic behavior is an intrinsic property of normal fecal microbiota and should be accounted for in comparing microbial communities among normal individuals and those with disease states. This also suggests that more frequent sample analyses are needed in order to properly assess success of FMT procedures. The online version of this article (doi:10.1186/s40168-015-0070-0) contains supplementary material, which is available to authorized users.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
11
作者：
Fuentes, Susana;van Nood, Els;de Vos, Willem M.
通讯作者：
de Vos, Willem M.
影响因子：
9.2
作者：
McDonald D;Clemente JC;Kuczynski J;Rideout JR;Stombaugh J;Wendel D;Wilke A;Huse S;Hufnagle J;Meyer F;Knight R;Caporaso JG
通讯作者：
Caporaso JG
影响因子：
7
作者：
Lozupone CA;Stombaugh J;Gonzalez A;Ackermann G;Wendel D;Vázquez-Baeza Y;Jansson JK;Gordon JI;Knight R
通讯作者：
Knight R
影响因子：
30.3
作者：
Gevers D;Kugathasan S;Denson LA;Vázquez-Baeza Y;Van Treuren W;Ren B;Schwager E;Knights D;Song SJ;Yassour M;Morgan XC;Kostic AD;Luo C;González A;McDonald D;Haberman Y;Walters T;Baker S;Rosh J;Stephens M;Heyman M;Markowitz J;Baldassano R;Griffiths A;Sylvester F;Mack D;Kim S;Crandall W;Hyams J;Huttenhower C;Knight R;Xavier RJ
通讯作者：
Xavier RJ