Analysis of Draft Genome Sequence of Pseudomonas sp. QTF5 Reveals Its Benzoic Acid Degradation Ability and Heavy Metal Tolerance.
Analysis of Draft Genome Sequence of Pseudomonas sp. QTF5 Reveals Its Benzoic Acid Degradation Ability and Heavy Metal Tolerance.
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基因组序列草案分析
DOI：
10.1155/2017/4565960
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发表时间：
2017
影响因子：
--
通讯作者：
Jiang N
中科院分区：
文献类型：
--
作者：
Li Y;Ren Y;Jiang N
假单胞菌QTF 5是从青藏高原羌塘盆地（海拔5，111 m）沥青层附近的连续多年冻土中分离到的。它具有耐冷性，对重金属具有高度和广泛的耐受性，并具有代谢苯甲酸和水杨酸的能力。为了深入了解其适应的遗传基础，我们进行了全基因组测序，并分析了抗性基因和代谢途径。基于已发表的31个假单胞菌属的120个基因组序列，计算机基因组DNA-DNA杂交（<54%）和平均核苷酸同源性计算（<94%）表明，QTF 5与亚麻假单胞菌（Pseudomonaslini）的亲缘关系最近，应被划分为一个新的种。本研究为进一步鉴定该菌适应极端环境的特异性机制及其在环境保护中的应用提供了遗传学基础。
Pseudomonas sp. QTF5 was isolated from the continuous permafrost near the bitumen layers in the Qiangtang basin of Qinghai-Tibetan Plateau in China (5,111 m above sea level). It is psychrotolerant and highly and widely tolerant to heavy metals and has the ability to metabolize benzoic acid and salicylic acid. To gain insight into the genetic basis for its adaptation, we performed whole genome sequencing and analyzed the resistant genes and metabolic pathways. Based on 120 published and annotated genomes representing 31 species in the genus Pseudomonas, in silico genomic DNA-DNA hybridization (<54%) and average nucleotide identity calculation (<94%) revealed that QTF5 is closest to Pseudomonas lini and should be classified into a novel species. This study provides the genetic basis to identify the genes linked to its specific mechanisms for adaptation to extreme environment and application of this microorganism in environmental conservation.
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影响因子：
14.9
作者：
Benson DA;Cavanaugh M;Clark K;Karsch-Mizrachi I;Lipman DJ;Ostell J;Sayers EW
通讯作者：
Sayers EW
DOI：
10.1073/pnas.0409727102
发表时间：
2005-02-15
影响因子：
11.1
作者：
Konstantinidis, KT;Tiedje, JM
通讯作者：
Tiedje, JM
影响因子：
3.8
作者：
Evangelisti, Luca;Tang, Shouyuan;Caminati, Walther
通讯作者：
Caminati, Walther
影响因子：
10.4
作者：
He, Jianglin;Wang, Jian;Chen, Yanting
通讯作者：
Chen, Yanting
影响因子：
3.1
作者：
Nhi-Cong, Le Thi;Mikolasch, Annett;Schauer, Frieder
通讯作者：
Schauer, Frieder