A resource of Cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex.
A resource of Cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex.
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DOI：
10.1016/j.neuron.2011.07.026
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发表时间：
2011-09-22
期刊：
影响因子：
16.2
通讯作者：
Huang ZJ
中科院分区：
文献类型：
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作者：
Taniguchi H;He M;Wu P;Kim S;Paik R;Sugino K;Kvitsiani D;Fu Y;Lu J;Lin Y;Miyoshi G;Shima Y;Fishell G;Nelson SB;Huang ZJ
了解大脑皮层神经回路的一个关键障碍是解开 GABA 能中间神经元的多样性。这种多样性给神经回路分析提出了一般性问题：这些中间神经元细胞类型是如何生成并组装成刻板的局部回路的，以及它们如何对构成从感知到认知等皮层功能的回路操作做出不同的贡献？利用小鼠基因工程，我们生成并表征了约 20 个 Cre 和可诱导的 CreER 敲入驱动系，这些驱动系可靠地靶向 GABA 能神经元的主要类别和谱系。 Cre 和 Flp 驱动程序的交集捕获了更多选定人群。基因靶向可以可靠地识别、监测和操纵皮质 GABA 能神经元，从而实现从细胞命运规范、迁移和连接到其在网络动态和行为中的功能的系统和全面的分析。因此，这种方法将加速整个哺乳动物大脑中 GABA 能回路的研究。
A key obstacle to understanding neural circuits in the cerebral cortex is that of unraveling the diversity of GABAergic interneurons. This diversity poses general questions for neural circuit analysis: how are these interneuron cell types generated and assembled into stereotyped local circuits and how do they differentially contribute to circuit operations that underlie cortical functions ranging from perception to cognition? Using genetic engineering in mice, we have generated and characterized ~20 Cre and inducible CreER knockin driver lines that reliably target major classes and lineages of GABAergic neurons. More select populations are captured by intersection of Cre and Flp drivers. Genetic targeting allows reliable identification, monitoring, and manipulation of cortical GABAergic neurons, thereby enabling a systematic and comprehensive analysis from cell fate specification, migration, and connectivity, to their functions in network dynamics and behavior. As such, this approach will accelerate the study of GABAergic circuits throughout the mammalian brain.
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