Regulation of mRNA encoding low density lipoprotein receptor and high density lipoprotein-binding protein in ovine corpora lutea.
Regulation of mRNA encoding low density lipoprotein receptor and high density lipoprotein-binding protein in ovine corpora lutea.
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绵羊黄体中编码低密度脂蛋白受体和高密度脂蛋白结合蛋白的 mRNA 的调节。
DOI：
10.1071/rd9961107
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Niswender,GD
中科院分区：
文献类型：
--
作者：
Tandeski,TR;Juengel,JL;Nett,TM;Niswender,GD
进行了三个实验来检查黄体中编码绵羊低密度脂蛋白受体（LDL-R）和高密度脂蛋白结合蛋白（HBP）的 mRNA 稳态浓度的调节。在实验1中，在发情周期的第3、6、9、12和15天（第0天，发情）从母羊身上收集黄体。还在动情周期的第 6、9、12 和 15 天获得了小和大类固醇生成黄体细胞的富集制剂。在实验2中，16只母羊在发情周期第5天被切除垂体并接受盐水、黄体生成素(LH)、生长激素(GH)或LH+GH的组合，直到在发情周期第12天收集黄体组织。还在发情周期第 5 天和第 12 天从垂体完整的对照母羊身上收集黄体。在实验3中，对发情周期第11天或第12天的13只母羊施用前列腺素F2α(PGF2α)，并在4小时、12小时和24小时后收集黄体。还从 4 只未注射的母羊和 4 只注射盐水（24 小时）的母羊身上收集了黄体。结果表明，编码 LDL-R 的 mRNA 浓度在整个动情周期中没有变化。发情周期第 3 天收集的黄体组织比发情周期任何其他天收集的黄体组织具有更高浓度的编码 HBP 的 mRNA。垂体切除术增加了编码 LDL-R 的 mRNA 浓度，但对编码 HBP 的 mRNA 浓度没有影响。 PGF2α注射后12小时，编码LDL-R的mRNA浓度降低，但编码HBP的mRNA浓度增加。注射 PGF2 α 后 24 小时，LDL-R 和 HBP mRNA 的浓度均降低。因此，黄体营养激素和溶黄体激素对黄体孕酮分泌的长期正向和急性负向调节不是由编码LDL-R或HBP的mRNA稳态浓度的变化介导的。
Three experiments were conducted to examine the regulation of steady-state concentrations of mRNA encoding ovine low density lipoprotein receptor (LDL-R) and high density lipoprotein-binding protein (HBP) in corpora lutea. In Experiment 1, corpora lutea were collected from ewes on Days 3, 6, 9, 12 and 15 (Day 0, oestrus) of the oestrous cycle. Enriched preparations of small and large steroidogenic luteal cells were also obtained on Days 6, 9, 12 and 15 of the oestrous cycle. In Experiment 2, 16 ewes were hypophysectomized on Day 5 of the oestrous cycle and received saline, luteinizing hormone (LH), growth hormone (GH) or a combination of LH+GH until collection of luteal tissue on Day 12 of the oestrous cycle. Corpora lutea were also collected from pituitary-intact control ewes on Day 5 and Day 12 of the oestrous cycle. In Experiment 3, 13 ewes on Day 11 or Day 12 of the oestrous cycle were administered prostaglandin F2 alpha (PGF2 alpha) and corpora lutea were collected 4 h, 12 h and 24 h later. Corpora lutea were also collected from 4 non-injected and 4 saline-injected (at 24 h) ewes. Results demonstrated that concentrations of mRNA encoding LDL-R did not differ throughout the oestrous cycle. Luteal tissue collected on Day 3 of the oestrous cycle had higher concentrations of mRNA encoding HBP than luteal tissue collected on any other day of the oestrous cycle. Hypophysectomy increased concentrations of mRNA encoding LDL-R but had no effect on concentrations of mRNA encoding HBP. Twelve hours following PGF2 alpha injection concentrations of mRNA encoding LDL-R were decreased but concentrations of mRNA encoding HBP were increased. Concentrations of both LDL-R and HBP mRNA were decreased 24 h following injection of PGF2 alpha. Thus, long-term positive and acute negative regulation of progesterone secretion from the corpus luteum by luteotrophic and luteolytic hormones was not mediated by changes in steady-state concentrations of mRNA encoding LDL-R or HBP.
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