Open-3DSIM: an open-source three-dimensional structured illumination microscopy reconstruction platform.
Open-3DSIM: an open-source three-dimensional structured illumination microscopy reconstruction platform.
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DOI:
10.1038/s41592-023-01958-0
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发表时间:
2023-08
期刊:
影响因子:
48
通讯作者:
Xi, Peng
中科院分区:
文献类型:
--
作者:
Cao, Ruijie;Li, Yaning;Chen, Xin;Ge, Xichuan;Li, Meiqi;Guan, Meiling;Hou, Yiwei;Fu, Yunzhe;Xu, Xinzhu;Jiang, Shan;Gao, Baoxiang;Xi, Peng
Open-3DSIM is an open-source reconstruction platform for three-dimensional structured illumination microscopy. We demonstrate its superior performance for artifact suppression and high-fidelity reconstruction relative to other algorithms on various specimens and over a range of signal-to-noise levels. Open-3DSIM also offers the capacity to extract dipole orientation, paving a new avenue for interpreting subcellular structures in six dimensions (xyzθλt). The platform is available as MATLAB code, a Fiji plugin and an Exe application to maximize user-friendliness. Open-3DSIM is a versatile open-source software for high-fidelity reconstruction of three-dimensional structured illumination microscopy data (with polarization). It is available in three convenient forms for user-friendly and customizable applications.
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