Poritiphilus flavus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from coral Porites lutea.

Poritiphilus flavus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from coral Porites lutea.
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黄波氏菌属

DOI:
10.1099/ijsem.0.004452
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发表时间:
2020-09
影响因子:
2.8
通讯作者:
Yu Kefu
Yu Kefu
中科院分区:
生物学3区
文献类型:
--
作者:
Wang Guanghua;Xu Shuailiang;Dang Ge;Liu Jianfeng;Su Hongfei;Chen Biao;Liao Zhiheng;Huang Wen;Liang Jiayuan;Wang Yinghui;Yu Kefu

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从珊瑚黄体Porites lutea中分离到一株革兰氏阴性、非孢子内形成、非运动、需氧细菌(菌株R33T),并进行了多相分类研究。G+C含量为44.5 mol%。唯一检出的呼吸醌为甲基萘醌6 (MK-6)。主要的细胞脂肪酸是iso-C15: 0和iso-C15: 1 ω6c。主要极性脂质为磷脂酰乙醇胺和两种未识别的脂质。基于16S rRNA基因序列的全局比对结果表明,菌株R33T与菌株R33T的同源性最高,为93.2%
A novel Gram-stain-negative, non-endospore-forming, non-motile, aerobic bacterium (strain R33T) was isolated from coral Porites lutea and subjected to a polyphasic taxonomic study. The G+C content was 44.5 mol%. The only detected respiratory quinone was menaquinone 6 (MK-6). The major cellular fatty acids were iso-C15 : 0 and iso-C15 : 1 ω6c. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. Global alignment based on 16S rRNA gene sequences indicated that strain R33T shares the highest sequence identity of 93.2 % with Muriicola marianensis A6B8T in the family Flavobacteriaceae . Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R33T forms a distinct branch in a stable clade comprising strain R33T and members of the genera Muriicola , Robiginitalea , Eudoraea and Zeaxanthinibacter . The phylogenomic analysis also supported this 16S rRNA gene-based phylogenetic result. Comparative genomic analysis indicated that strain R33T is rich in AraC-type DNA-binding domain-containing protein-coding genes, which means the regulation of carbon utilization is very complex. Low 16S rRNA gene identity, different polar lipids and/or cellular fatty acid profiles could readily distinguish strain R33T from any validly published type strains. Therefore, strain R33T is suggested to represent a new species in a new genus, for which the name Poritiphilus flavus gen. nov., sp. nov. is proposed. The type strain is R33T (=MCCC 1K03853T=KCTC 72443T).
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