Dissection of Interaction Kinetics through Single-Molecule Interaction Simulation.
Dissection of Interaction Kinetics through Single-Molecule Interaction Simulation.
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DOI：
10.1021/acs.analchem.0c01014
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发表时间：
2020-09-01
影响因子：
7.4
通讯作者：
Chen TY
中科院分区：
文献类型：
--
作者：
Pan M;Zhang Y;Yan G;Chen TY
The ability to extract kinetic interaction parameters from single-molecule fluorescence resonance energy transfer trajectories without the need for solving complex single-molecule differential equations has the potential to address some of the critical biophysical questions. Here, we provide a single-molecule interaction simulation (SMIS) tool to give the expected dwell-time distributions and relative populations of each FRET states based on the assigned kinetic model and to dissect kinetic interaction parameters from single-molecule FRET trajectories. The method provides the expected dwell-time distributions, averaged transition rates, and relative populations of each FRET states based on the assigned kinetic model. Comparing extensive simulated data with experimental data enables the quantification of the kinetic rate and equilibrium constants. We have also demonstrated that SMIS is useful to quantify the interaction kinetic rate constants that were originally unobtainable through the traditional single-molecule analytical solution approach.
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影响因子：
16.6
作者：
Chen TY;Santiago AG;Jung W;Krzemiński Ł;Yang F;Martell DJ;Helmann JD;Chen P
通讯作者：
Chen P
影响因子：
3
作者：
Brunger, Axel T.;Strop, Pavel;Vrljic, Marija;Chu, Steven;Weninger, Keith R.
通讯作者：
Weninger, Keith R.
影响因子：
6.8
作者：
Danial, John S. H.;Garcia-Saez, Ana J.
通讯作者：
Garcia-Saez, Ana J.
DOI：
10.1126/science.1215768
发表时间：
2012-02-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chung HS;McHale K;Louis JM;Eaton WA
通讯作者：
Eaton WA
影响因子：
2.3
作者：
Haran, G
通讯作者：
Haran, G