The application of on-chip optofluidic microscopy for imaging Giardia lamblia trophozoites and cysts.
The application of on-chip optofluidic microscopy for imaging Giardia lamblia trophozoites and cysts.
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DOI：
10.1007/s10544-009-9312-x
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发表时间：
2009-10
影响因子：
2.8
通讯作者：
Yang, Changhuei
中科院分区：
文献类型：
--
作者：
Lee, Lap Man;Cui, Xiquan;Yang, Changhuei
光流控显微镜 (OFM) 是一种无透镜、低成本且高度紧凑的片上设备，可以实现高分辨率显微镜成像。 OFM 通过在斜孔阵列上流动/扫描目标物体来进行成像；通过测量通过孔的随时间变化的光传输变化，我们可以以与孔尺寸相当的分辨率渲染目标物体的图像。本文报道了 OFM 对蓝氏贾第鞭毛虫滋养体和包囊成像的适应情况，兰氏贾第鞭毛虫滋养体和包囊是一种常见于劣质水源中的致病寄生虫。我们还描述了基于压力的流动和基于直流电动的流动在控制贾第鞭毛虫包囊流动运动中的影响的研究——寄生虫的无旋转平移对于良好的 OFM 图像采集非常重要。最后，我们报告了使用焦平面分辨率为 0.8 微米的 OFM 对贾第鞭毛虫滋养体和包囊进行的成功显微镜成像。
The optofluidic microscope (OFM) is a lensless, low-cost and highly compact on-chip device that can enable high-resolution microscopy imaging. The OFM performs imaging by flowing/scanning the target objects across a slanted hole array; by measuring the time-varying light transmission changes through the holes, we can then render images of the target objects at a resolution that is comparable to the holes' size. This paper reports the adaptation of the OFM for imaging Giardia lamblia trophozoites and cysts, a disease-causing parasite species that is commonly found in poor-quality water sources. We also describe our study of the impact of pressure-based flow and DC electrokinetic-based flow in controlling the flow motion of Giardia cysts – rotation-free translation of the parasite is important for good OFM image acquisition. Finally, we report the successful microscopy imaging of both Giardia trophozoites and cysts with an OFM that has a focal plane resolution of 0.8 microns.
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影响因子：
7.4
作者：
Hu, SW;Ren, XQ;Allbritton, N
通讯作者：
Allbritton, N
DOI：
10.1073/pnas.0804612105
发表时间：
2008-08-05
影响因子：
11.1
作者：
Cui, Xiquan;Lee, Lap Man;Yang, Changhuei
通讯作者：
Yang, Changhuei
影响因子：
6.1
作者：
Heng, Xin;Erickson, David;Yang, Changhuei
通讯作者：
Yang, Changhuei
影响因子：
6.1
作者：
Lay, Christophe;Teo, Cheng Yong;Liu, Wen-Tso
通讯作者：
Liu, Wen-Tso
影响因子：
2.2
作者：
Sakamoto, Chieko;Yamaguchi, Nobuyasu;Nasu, Masao
通讯作者：
Nasu, Masao