The spatial distribution of glutamatergic inputs to dendrites of retinal ganglion cells.
The spatial distribution of glutamatergic inputs to dendrites of retinal ganglion cells.
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DOI：
10.1002/cne.21795
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发表时间：
2008-09-10
影响因子：
2.5
通讯作者：
Masland, Richard H.
中科院分区：
文献类型：
--
作者：
Jakobs, Tatjana C.;Koizumi, Amane;Masland, Richard H.
关键词：
利用PSD95-GFP表达质粒的颗粒介导基因转移，在兔视网膜的大量神经节细胞中可视化了兴奋性谷氨酸能输入的空间模式。PSD95-GFP通过与突触带（RIBEYE和kinesin II）和谷氨酸受体亚基共定位而被证实是兴奋性输入的标记。尽管细胞的大小、形态和功能复杂性存在很大差异，但兴奋性突触输入的分布遵循一套单一的规则：(1)突触输入的线性密度（PSD95位点/线性μm）变化惊人地小，并且在树体内几乎没有表现出专业化。(2)各乔木间兴奋性输入的总密度在胞体周围的环状区域达到峰值。这与兔子感受野敏感度的高分辨率图一致。(3)面密度与树突乔木的总面积成反比，单位面积上狭窄的树突乔木比大的树突乔木接收到更多的突触。为了获得与大细胞相当的灵敏度，那些报告视觉空间小区域的细胞可能需要从该空间内接收更密集的突触输入。
The spatial pattern of excitatory glutamatergic input was visualized in a large series of ganglion cells of the rabbit retina, using particle-mediated gene transfer of an expression plasmid for PSD95-GFP. PSD95-GFP was confirmed as a marker of excitatory input by co-localization with synaptic ribbons (RIBEYE and kinesin II) and glutamate receptor subunits. Despite wide variation in the size, morphology, and functional complexity of the cells, the distribution of excitatory synaptic inputs followed a single set of rules: (1) The linear density of synaptic inputs (PSD95 sites / linear μm) varied surprisingly little and showed little specialization within the arbor. (2) The total density of excitatory inputs across individual arbors peaked in a ring-shaped region surrounding the soma. This is in accord with high-resolution maps of receptive field sensitivity in the rabbit. (3) The areal density scaled inversely with the total area of the dendritic arbor, so that narrow dendritic arbors receive more synapses per unit area than large ones. To achieve sensitivity comparable to that of large cells, those that report upon a small region of visual space may need to receive a denser synaptic input from within that space.
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