Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty.
Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty.
复制标题
青春期大鼠弓状核转录组规模的空间基因表达
DOI：
10.1186/s13578-022-00745-2
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发表时间：
2022-01-21
影响因子：
7.5
通讯作者：
Shen Y
中科院分区：
文献类型：
--
作者：
Zhou S;Zang S;Hu Y;Shen Y;Li H;Chen W;Li P;Shen Y
青春期下丘脑-垂体-性腺轴激活过程中，下丘脑内多种神经元的转录活性受到多个基因的复杂调控。鉴定青春期相关的细胞组成和表征不同细胞中独特的转录特征有利于分离特定的神经元和深入了解其功能。采用10× GenomicsVisium平台，对出生后第25、35和45天的雌性SD大鼠下丘脑弓状核（ARC）基因表达进行动态空间图谱构建。用kisspeptin神经元选择性表达的表面蛋白，通过流式细胞术对体外培养的神经元进行分选。使用Smart测序检查分离的细胞的转录组。在ARC中鉴定出具有相似基因表达特征的四个神经元亚群。只有一个亚群显示Kiss 1的稳健表达，其可以通过独特的膜表面生物标志物溶质载体家族18成员A3（SLC 18 A3）分离。此外，在不同的亚群基因呈现三个表达模块不同的功能，在每个青春期阶段。下丘脑-垂体-性腺轴的启动和调节是由不同类型的细胞在神经胶质细胞或神经元分化、激素分泌和雌二醇反应等方面的不同功能相互影响和协调而形成的复杂的调控网络。我们的数据揭示了一个全面的转录组学概述ARC在不同的青春期阶段，这可能是一个宝贵的资源，青春期和性发育障碍的研究。在线版本包含补充材料，可通过10.1186/s13578-022-00745-2获得。
A variety of neurons in hypothalamus undergo a complicated regulation on transcription activity of multiple genes for hypothalamic–pituitary–gonadal axis activation during pubertal development. Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to isolation of specific neurons and advanced understanding of their functions. The hypothalamus of female Sprague–Dawley rats in postnatal day-25, 35 and 45 were used to define the dynamic spatial atlas of gene expression in the arcuate nucleus (ARC) by 10× Genomics Visium platform. A surface protein expressed selectively by kisspeptin neurons was used to sort neurons by flow cytometric assay in vitro. The transcriptome of the isolated cells was examined using Smart sequencing. Four subclusters of neurons with similar gene expression signatures in ARC were identified. Only one subcluster showed the robust expression of Kiss1, which could be isolated by a unique membrane surface biomarker Solute carrier family 18 member A3 (SLC18A3). Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic–pituitary–gonadal axis initiation and modulation. Our data revealed a comprehensive transcriptomic overview of ARC within different pubertal stages, which could serve as a valuable resource for the study of puberty and sexual development disorders. The online version contains supplementary material available at 10.1186/s13578-022-00745-2.
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影响因子：
2.7
作者：
Barroso, Alexia;Roa, Juan;Tena-Sempere, Manuel
通讯作者：
Tena-Sempere, Manuel
DOI：
10.1210/me.2012-1371
发表时间：
2013-04
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Atkin SD;Owen BM;Bookout AL;Cravo RM;Lee C;Elias CF;Elmquist JK;Kliewer SA;Mangelsdorf DJ
通讯作者：
Mangelsdorf DJ
影响因子：
56.9
作者：
Stahl, Patrik L.;Salmen, Fredrik;Frisen, Jonas
通讯作者：
Frisen, Jonas
雌性大鼠下丘脑EAP1、CUX1的表达及其与KISS1、GnRH的关系
影响因子：
2
作者：
Xu, Jing;Li, Pin
通讯作者：
Li, Pin
影响因子：
3.7
作者：
Shen Y;Zhou S;Zhao X;Li H;Sun J
通讯作者：
Sun J