Experimental data on developmental elimination of retrosplenial cortex GABAergic interneurons in a mouse model of ethanol exposure during the last trimester of human pregnancy.
Experimental data on developmental elimination of retrosplenial cortex GABAergic interneurons in a mouse model of ethanol exposure during the last trimester of human pregnancy.
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DOI：
10.1016/j.dib.2022.108355
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发表时间：
2022-08
期刊：
影响因子：
1.2
通讯作者：
Valenzuela, C. Fernando
中科院分区：
文献类型：
--
作者：
Barber, Megan J.;McDonald, Casey D.;Chavez, Glenna J.;Bird, Clark W.;Valenzuela, C. Fernando
先前已经表明，在出生后发育的前两周期间，40%的小鼠皮质中间神经元通过凋亡被消除。在这里，我们报告的数据上的影响，乙醇暴露在这个过程中，在人类妊娠的最后三个月（相当于小鼠出生后的第一周），在小鼠模型的酗酒样酒精暴露。我们使用在囊泡GABA转运蛋白启动子控制下的GABA能中间神经元中表达Venus荧光蛋白的转基因小鼠（VGAT-Venus小鼠）。在出生后第4至9天，使用蒸汽吸入室将小鼠暴露于空气（对照）或乙醇中4小时/天。这种暴露模式产生的血液乙醇浓度峰值在300至400 mg/dl之间。在出生后第5、7、10和30天在麻醉下进行经心灌注。用荧光DNA染料4′6-diamidino-2-phenylindole（DAPI）对冷冻机制备的漂浮切片进行染色。然后，我们量化了静脉阳性GABA能中间神经元的密度在层I，II-IV和V的压后皮质，这是边缘记忆系统的一部分，是敏感的乙醇诱导的凋亡在小鼠出生后的第一周。这些数据表明，中间神经元的密度下降，在压后皮质层在第一周的生活和乙醇暴露并没有显着改变这一进程。这些数据可能对正在研究乙醇和其他致畸剂对大脑皮层发育中的中间神经元的影响的研究人员感兴趣。
It has been previously shown that 40% of murine cortical interneurons are eliminated via apoptosis during the first two weeks of postnatal development. Here, we report data on the effect of ethanol exposure on this process in a mouse model of binge-like alcohol exposure during last trimester of human pregnancy (equivalent to the first postnatal week in mice). We used transgenic mice that express the Venus fluorescent protein in GABAergic interneurons under the control of the vesicular GABA transporter promoter (VGAT-Venus mice). Mice were exposed to air (controls) or ethanol for 4 hr/day on postnatal days 4 to 9 using vapor inhalation chambers. This exposure paradigm produces peak blood ethanol concentrations between 300 and 400 mg/dl. Transcardial perfusions were performed under anesthesia at postnatal days 5, 7, 10 and 30. Cryostat-prepared floating sections were stained with the fluorescent DNA dye, 4′6-diamidino-2-phenylindole (DAPI). We then quantified the density of Venus-positive GABAergic interneurons in layers I, II-IV and V of the retrosplenial cortex, which is part of the limbic memory system, and is sensitive to ethanol-induced apoptosis during the first postnatal week in mice. The data show that density of interneurons decreases in the retrosplenial cortex layers during the first week of life and that ethanol exposure does not significantly alter this process. These data may be of interest to investigators who are studying the effect of ethanol and other teratogenic agents on developing interneurons in the cerebral cortex.
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影响因子：
--
作者：
Bubb, Emma J;Kinnavane, Lisa;Aggleton, John P
通讯作者：
Aggleton, John P
影响因子：
64.8
作者：
Wong FK;Bercsenyi K;Sreenivasan V;Portalés A;Fernández-Otero M;Marín O
通讯作者：
Marín O
影响因子：
3.3
作者：
Bird CW;Taylor DH;Pinkowski NJ;Chavez GJ;Valenzuela CF
通讯作者：
Valenzuela CF
影响因子：
4.7
作者：
Bird, Clark W.;Barber, Megan J.;Valenzuela, C. Fernando
通讯作者：
Valenzuela, C. Fernando
DOI：
10.1111/j.1530-0277.2007.00351.x
发表时间：
2007-04-01
影响因子：
3.2
作者：
Saito, Mariko;Mao, Rui-Fen;Saito, Mitsuo
通讯作者：
Saito, Mitsuo