Prevalence, identification of virulence factors, O-serogroups and antibiotic resistance properties of Shiga-toxin producing Escherichia coli strains isolated from raw milk and traditional dairy products.
Prevalence, identification of virulence factors, O-serogroups and antibiotic resistance properties of Shiga-toxin producing Escherichia coli strains isolated from raw milk and traditional dairy products.
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DOI：
10.1186/s13756-018-0345-x
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发表时间：
2018
影响因子：
5.5
通讯作者：
Rahimi E
中科院分区：
文献类型：
--
作者：
Ranjbar R;Safarpoor Dehkordi F;Sakhaei Shahreza MH;Rahimi E
志贺样大肠埃希菌是最重要的食源性细菌之一，具有耐药性。食源性STEC菌株主要与某些毒力因子和O-型群的存在相关。本研究对乳及乳制品中分离的志贺菌性大肠杆菌的毒力因子分布、O血清群及耐药性进行了研究。随机收集了600份样本，并立即转移到实验室。所有样品均进行培养，E.分离出大肠杆菌菌株。基于推定的毒力因子和亚型的存在来鉴定STEC菌株。对STEC分离株进行多重PCR和纸片扩散法。600份样品中有181份（30.16%）携带E.杆菌STEC感染率为10.66%。O 157（43.75%）和O26（37.50%）是最常见的血清群。Aac（3）-IV（100%）、CITM（96.87%）和tetA（76.56%）是最常见的耐药基因。STEC对氨苄西林、庆大霉素和四环素的耐药率最高，分别为100%、100%和96.87%。Kashk和dough均为阴性。大肠杆菌菌株。耐O 157菌株的高流行率和多种毒力因子的同时存在，对生奶和乳制品的消费构成了重要的公共卫生问题。
Shiga-toxigenic Escherichia coli strains are one of the most important foodborne bacteria with an emergence of antibiotic resistance. Foodborne STEC strains are mainly associated with presence of certain virulence factors and O-seogroups. The present investigation was done to study the distribution of virulence factors, O-serogroups and antibiotic resistance properties of Shiga-toxigenic Escherichia coli isolated from milk and dairy products. Six-hundred samples were randomly collected and immediately transferred to laboratory. All samples were cultured and E. coli strains were isolated. STEC strains were identified based on the presence of putative virulence factors and subtypes. STEC isolates were subjected to multiplex PCR and disk diffusion methods. One-hundred and eighty-one out of 600 samples (30.16%) harbored E. coli. Prevalence of STEC strains was 10.66%. O157 (43.75%) and O26 (37.50%) were the most frequently identified serogroups. Aac(3)-IV (100%), CITM (96.87%) and tetA (76.56%) were the most commonly detected antibiotic resistance genes. STEC strains had the highest prevalence of resistance against ampicillin (100%), gentamicin (100%) and tetracycline (96.87%). Kashk and dough were negative for presence of E. coli strains. High prevalence of resistant-O157 strains and simultaneous presence of multiple virulence factors pose an important public health problem regarding the consumption of raw milk and dairy products.
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影响因子：
0.4
作者：
Dormanesh B;Safarpoor Dehkordi F;Hosseini S;Momtaz H;Mirnejad R;Hoseini MJ;Yahaghi E;Tarhriz V;Khodaverdi Darian E
通讯作者：
Khodaverdi Darian E
影响因子：
1.8
作者：
Dehkordi FS;Yazdani F;Mozafari J;Valizadeh Y
通讯作者：
Valizadeh Y
影响因子：
5.5
作者：
Amézquita-López BA;Quiñones B;Soto-Beltrán M;Lee BG;Yambao JC;Lugo-Melchor OY;Chaidez C
通讯作者：
Chaidez C
影响因子：
0.6
作者：
Dehkordi, Farhad Safarpoor;Barati, Sara;Dehkordi, Shahin Nejat
通讯作者：
Dehkordi, Shahin Nejat
影响因子：
1.2
作者：
Behzadi P;Najafi A;Behzadi E;Ranjbar R
通讯作者：
Ranjbar R