A novel engineered purified exosome product patch for tendon healing: An explant in an ex vivo model.
A novel engineered purified exosome product patch for tendon healing: An explant in an ex vivo model.
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DOI：
10.1002/jor.24859
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发表时间：
2021-08
影响因子：
2.8
通讯作者：
Zhao, Chunfeng
中科院分区：
文献类型：
--
作者：
Shi, Guidong;Wang, Yicun;Wang, Zhanwen;Thoreson, Andrew R.;Jacobson, Daniel S.;Amadio, Peter C.;Behfar, Atta;Moran, Steven L.;Zhao, Chunfeng
由于肌腱本身较差的愈合能力，减少肌腱修复后的失效仍然是一个挑战。本研究的目的是研究一种新型组织工程纯化外泌体产品（PEP）贴片对犬离体模型肌腱愈合的影响。用Tisseel贴片或PEP生物增强剂对三只犬爪撕裂的指深屈肌腱进行模拟修复。离体模型将FDP肌腱随机分为3组：单独FDP肌腱修复组（Control）、单独Tisseel贴片组、Tisseel + PEP （TEPEP）贴片组。组织培养4周后，评估愈合肌腱的失效负荷、刚度、组织学和基因表达。透射电镜（TEM）显示PEP外泌体直径在93.70 ~ 124.65 nm之间，贴片释放试验表明，该TEPEP贴片能在2周内稳定释放细胞外囊泡。TEPEP贴片组肌腱的破坏强度明显高于对照组和单独使用Tisseel组。组织学结果显示，TEPEP贴片组损伤肌腱表面愈合间隙最小，成纤维细胞数量最多。定量RT-PCR结果显示，TEPEP patch提高了COL3、MMP2、MMP3、MMP14的表达，降低了tgf - β 1、IL-6的表达。本研究表明，TEPEP贴片可通过减少间隙形成和炎症反应，增加内源性细胞活性和III型胶原的形成来促进肌腱修复。
Reducing tendon failure after repair remains a challenge due to its poor intrinsic healing ability. The purpose of this study is to investigate the effect of a novel tissue-engineered purified exosome product (PEP) patch on tendon healing in a canine ex vivo model. Lacerated flexor digitorum profundus (FDP) tendons from three canines’ paws underwent simulated repair with Tisseel patch alone or biopotentiated with PEP. For ex vivo model, FDP tendons were randomly divided into 3 groups: FDP tendon repair alone group (Control), Tisseel patch alone group, and Tisseel plus PEP (TEPEP) patch group. Following four weeks of tissue culture, the failure load, stiffness, histology and gene expression of the healing tendon were evaluated. The transmission electron microscopy (TEM) revealed that exosomes of PEP the diameters ranged from 93.70 to 124.65 nm, and the patch release test showed this TEPEP patch could stably release the extracellular vesicle over two weeks. The failure strength of tendon in the TEPEP patch group was significantly higher than that of the Control group and Tisseel alone group. The results of histology showed that the TEPEP patch group had the smallest healing gap and the largest number of fibroblasts on the surface of the injured tendon. Quantitative RT-PCR showed that TEPEP patch increased the expression of COL3, MMP2, MMP3, MMP14, and reduced the expression of TGF-beta1, IL-6. This study shows that the TEPEP patch could promote tendon repair by reducing gap formation and inflammatory response, increasing the activity of endogenous cells and the formation of type III collagen.
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影响因子：
8.8
作者：
Cianciaruso, Chiara;Beltraminelli, Tim;De Palma, Michele
通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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DOI：
10.1080/21691401.2017.1304408
发表时间：
2018-01-01
影响因子：
5.8
作者：
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影响因子：
11.5
作者：
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通讯作者：
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