Organ-on-a-Chip Platforms for Drug Screening and Delivery in Tumor Cells: A Systematic Review.
Organ-on-a-Chip Platforms for Drug Screening and Delivery in Tumor Cells: A Systematic Review.
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肿瘤细胞中药物筛查和输送的器官芯片平台：系统评价。
DOI：
10.3390/cancers14040935
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发表时间：
2022-02-13
期刊：
影响因子：
5.2
通讯作者：
Minas G
中科院分区：
文献类型：
--
作者：
Gonçalves IM;Carvalho V;Rodrigues RO;Pinho D;Teixeira SFCF;Moita A;Hori T;Kaji H;Lima R;Minas G
癌症是世界范围内死亡率很高的疾病之一。在目前研究新的诊断和治疗工具的策略中，器官芯片在实现更个性化的医学方面是非常有前途的。在这项工作中，从820篇最新发表的科学文章中选出75篇，并通过系统的过程进行分析。所选文章介绍了不同的微流体平台，其中引入了细胞培养物，并用于评估癌症治疗的疗效和/或毒性。发展癌症模型，纠正单层或静态细胞培养生理微环境的简单性，同时比动物模型更准确地复制人类系统，一直是生物医学研究中的一个挑战。器官芯片（OoC）设备是过去十年来一直在探索的解决方案。微流体和细胞培养的组合允许设计适合于评估治疗功效和效果的动态微环境，更接近于在患者中观察到的反应。本系统综述总结了过去十年的研究，其中OoC与癌细胞培养物用于药物筛选测定。这些研究选自三个数据库，并按照PRISMA提出的系统评价研究指南进行分析。在选定的研究中，评估了几种类型的癌细胞，并且测试的大多数治疗是标准化疗药物。一些研究报告了OoC装置上的培养物的耐药性高于2D培养物，这表明前者与体内条件的相似性更好。几项研究还包括微血管的复制或不同细胞培养物的组合。脉管系统的存在可以积极或消极地影响药物功效，因为它有助于药物以及氧气和营养物的更大扩散。与肝细胞共培养有助于评价某些药物代谢产物的全身毒性。然而，很少有研究使用患者细胞进行药物筛选测定。
Cancer is one of the diseases with a high mortality rate worldwide. Of the current strategies to study new diagnostic and treating tools, organs-on-chip are quite promising regarding the achievement of more personalized medicine. In this work, 75 out of 820 of the most recent published scientific articles were selected and analyzed through a systematic process. The selected articles present the different microfluidic platforms where cell culture was introduced and was used for the evaluation of cancer treatments efficacy and/or toxicity. The development of cancer models that rectify the simplicity of monolayer or static cell cultures physiologic microenvironment and, at the same time, replicate the human system more accurately than animal models has been a challenge in biomedical research. Organ-on-a-chip (OoC) devices are a solution that has been explored over the last decade. The combination of microfluidics and cell culture allows the design of a dynamic microenvironment suitable for the evaluation of treatments’ efficacy and effects, closer to the response observed in patients. This systematic review sums the studies from the last decade, where OoC with cancer cell cultures were used for drug screening assays. The studies were selected from three databases and analyzed following the research guidelines for systematic reviews proposed by PRISMA. In the selected studies, several types of cancer cells were evaluated, and the majority of treatments tested were standard chemotherapeutic drugs. Some studies reported higher drug resistance of the cultures on the OoC devices than on 2D cultures, which indicates the better resemblance to in vivo conditions of the former. Several studies also included the replication of the microvasculature or the combination of different cell cultures. The presence of vasculature can influence positively or negatively the drug efficacy since it contributes to a greater diffusion of the drug and also oxygen and nutrients. Co-cultures with liver cells contributed to the evaluation of the systemic toxicity of some drugs metabolites. Nevertheless, few studies used patient cells for the drug screening assays.
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在药物发现的边界，片上的器官。
DOI：
10.1038/nrd4539
发表时间：
2015-04
期刊：
Nature reviews. Drug discovery
影响因子：
--
作者：
Esch EW;Bahinski A;Huh D
通讯作者：
Huh D
影响因子：
3.9
作者：
Chen, Pin-Yao;Hsieh, Meng-Ju;Hou, Yung-Te
通讯作者：
Hou, Yung-Te
影响因子：
7.4
作者：
Ghaemmaghami, Amir M.;Hancock, Matthew J.;Harrington, Helen;Kaji, Hirokazu;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
4.2
作者：
Flont, Magdalena;Jastrzebska, Elzbieta;Brzozka, Zbigniew
通讯作者：
Brzozka, Zbigniew
用于细胞共培养和药物代谢模拟的集成微流体系统
影响因子：
3.9
作者：
Jie, Mingsha;Li, Hai-Fang;Lin, Jin-Ming
通讯作者：
Lin, Jin-Ming