Improvement of hypocrellin production by a new fungal source and optimization of cultivation conditions
Improvement of hypocrellin production by a new fungal source and optimization of cultivation conditions
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新真菌来源提高竹红菌素产量及优化培养条件
DOI：
10.1080/13102818.2016.1178077
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发表时间：
2016-05
期刊：
影响因子：
--
通讯作者：
Shen XY Hou CL
中科院分区：
文献类型：
--
作者：
Shen XY Hou CL
竹红菌素被认为是光动力疗法中新一代有潜力的光敏剂。然而，目前大量生产HA是有限的，无论是从Hypcrella besthiae和Shiraia besthicola的子实体提取，还是其发酵菌丝体。为突破这一瓶颈，本研究从毛竹种子中分离筛选出350多株真菌，获得14株白菌。bacillicola，然而，我们研究中的竹红菌素产生菌株被鉴定为内生真菌，其中几种菌株表现出显著高含量的竹红菌素。其中，一株新分离的Shiraia sp.菌株ZZZ 816被选作进一步研究。采用单因素试验和正交试验设计，对菌株ZZZ 816液体发酵生产竹红菌素的条件进行了优化。结果表明，液体发酵的最佳工艺条件为：培养时间144 h，初始pH 6.0，装液量100/250 mL，转速130 rpm，菌龄60 h，接种量10%。最佳培养基组成为：酵母膏20 g/L，麦芽糖40 g/L，FeSO 4·H2O 0.5g/L，尿素4.0g/L，MgSO 4·7 H2O 0.5g/L，初始pH 5.0。采用最佳生产条件进行深层发酵，菌株ZZZ 816的竹红菌甲素含量最高可达921.6mg/L，高于以往报道的其他工业菌株。结果表明，菌株ZZZ 816通过优化发酵工艺，可显著提高竹红菌素的产量。
ABSTRACT Hypocrellins is regarded as the new generation of potential photosensitizer in the photodynamic therapy. However, the current production of HA in large quantity is limited, whether extracted from the fruit bodies of Hypcrella bambusae and Shiraia bambusicola, or its fermented mycelia. To break through the bottleneck, we screened over 350 fungal strains from the uncommon moso bamboo (Phyllostachys edulis) seeds, and acquired 14 isolates of Shiraia sp. Hypocrellins were generally extracted from the stroma of S. bambusicola, and however the hypocrellin-producing strains in our study were identified as endophytic fungi, where several of them exhibited significantly high content of hypocrellins. One of them, a newly isolated Shiraia sp. strain ZZZ816 was selected for further research. The hypocrellin production conditions of the strain ZZZ816 under submerged fermentation were optimized by one-factor-at-a-time and orthogonal experiment design. The optimum parameters for the quid fermentation were determined as follows: incubation time (144 h), initial pH (6.0), volume of medium (100/250 mL), rotary speed (130 rpm), mycelial age (60 h) and inoculation level (10%). The optimum culture medium constituents were determined as follows: yeast extract 20g/L, malt sugar 40g/L, FeSO4·H2O 0.5g/L, urea 4.0g/L, MgS04·7H20 0.5g/L and initial pH 5.0. Using submerged fermentation of the optimum production conditions, the maximum content of hypocrellin A from the strain ZZZ816 was 921.6 mg/L, more than the other industrial strains published in the previous reports. The results demonstrated that Shiraia sp. strain ZZZ816 could enhance the hypocrellin production tremendously with optimizing the fermentation progress.
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DOI：
10.1111/j.1365-2621.2009.02080.x
发表时间：
2009-12
影响因子：
3.3
作者：
Yujie Su;Xiaoyan Yin;Shengqi Rao;Yujie Cai;B. Reuhs;Yan-jun Yang
通讯作者：
Yujie Su;Xiaoyan Yin;Shengqi Rao;Yujie Cai;B. Reuhs;Yan-jun Yang
影响因子：
3.3
作者：
Yujie Su;Shaohong Si;Liwen Qiao;Yujie Cai;Zhimin Xu;Yan-jun Yang
通讯作者：
Yujie Su;Shaohong Si;Liwen Qiao;Yujie Cai;Zhimin Xu;Yan-jun Yang
影响因子：
--
作者：
X. Ling
通讯作者：
X. Ling
影响因子：
3.3
作者：
Diwu Zhenjun;J. Lown
通讯作者：
Diwu Zhenjun;J. Lown
影响因子：
4.9
作者：
GARDES, M;BRUNS, TD
通讯作者：
BRUNS, TD