Identification of a novel bacteriocin regulatory system in Streptococcus mutans.
Identification of a novel bacteriocin regulatory system in Streptococcus mutans.
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DOI：
10.1111/j.1365-2958.2010.07417.x
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发表时间：
2010-12
影响因子：
3.6
通讯作者：
Merritt J
中科院分区：
文献类型：
--
作者：
Xie Z;Okinaga T;Niu G;Qi F;Merritt J
最近，我们描述了一个未表征的两个基因的调控系统的功能，由一个LytTR家族的转录调节因子和一个假定的膜蛋白，我们称之为hdrRM操纵子。在这项研究中，我们确定HdrRM系统控制一个类似的未表征的监管系统的表达，注释为SMU.2080和SMU.2081。与hdrRM一样，SMU.2080-2081操纵子编码LytTR家族转录调节因子和推定的膜蛋白，我们现在分别将其称为BrsR和BrsM。BrsRM系统的调节机制的检查表明，BrsM用于拮抗转录调节剂BrsR的功能。对BrsR的调节作用的进一步分析确定其作为多种细菌素和细菌素相关基因的转录激活剂发挥作用。在体外电迁移率变动测定证实，BrsR结合到几个细菌素基因的启动子区，并需要一个LytTR家族的共识直接重复序列的存在下，以稳定地结合DNA。此外，我们确定了一种新的监管方案，其中HdrRM和BrsRM系统相互调节，并最终确定细菌素生产是否会抑制竞争对手的生物体或导致对生产者的致命性。
Recently, we described the function of an uncharacterized two-gene regulatory system consisting of a LytTR family transcription regulator and a putative membrane protein, which we referred to as the hdrRM operon. In this study, we determined that the HdrRM system controls the expression of an analogous uncharacterized regulatory system annotated as SMU.2080 and SMU.2081. Like hdrRM, the SMU.2080–2081 operon encodes a LytTR family transcription regulator and putative membrane protein, which we now refer to as BrsR and BrsM, respectively. Examination of the regulatory mechanism of the BrsRM system suggests that BrsM serves to antagonize the function of the transcription regulator BrsR. Further analyses of the regulatory role of BrsR determined that it functions as a transcription activator for a variety of bacteriocins and bacteriocin-related genes. In vitro electromobility shift assays confirmed that BrsR binds to the promoter regions of several bacteriocin genes and requires the presence of a LytTR family consensus direct repeat in order to stably bind DNA. In addition, we identified a novel regulatory scheme in which both the HdrRM and BrsRM systems coregulate each other and ultimately determine whether bacteriocin production will inhibit competitor organisms or result in lethality to the producer.
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影响因子：
3.2
作者：
Kreth, Jens;Zhang, Yongshu;Herzberg, Mark C.
通讯作者：
Herzberg, Mark C.
影响因子：
4.2
作者：
dos Santos, MN;dos Santos, LM;Cury, JA
通讯作者：
Cury, JA
影响因子：
4.4
作者：
Hale, JDF;Ting, YT;Heng, NCK
通讯作者：
Heng, NCK
影响因子：
7.6
作者：
BOWDEN, GHW
通讯作者：
BOWDEN, GHW
影响因子：
3.2
作者：
Kreth, J;Merritt, J;Qi, FX
通讯作者：
Qi, FX