Diagnostic efficiency of RPA/RAA integrated CRISPR-Cas technique for COVID-19: A systematic review and meta-analysis.
Diagnostic efficiency of RPA/RAA integrated CRISPR-Cas technique for COVID-19: A systematic review and meta-analysis.
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RPA/RAA 集成 CRISPR-Cas 技术对 COVID-19 的诊断效率：系统评价和荟萃分析
DOI：
10.1371/journal.pone.0276728
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Li, Heng
中科院分区：
文献类型：
--
作者：
Zhang, Xiaoyu;Ge, Xiao;Shen, Fangyuan;Qiao, Jinjuan;Zhang, Yubo;Li, Heng
目的探讨重组酶聚合酶/辅助扩增(RPA/RAA)技术在SARS冠状病毒2型(SARS CoV 2)诊断中的应用价值。应用计算机检索新冠肺炎相关文献，检索词为“新型冠状病毒”、“规律性短回文重复序列”和“RPA/RAA”，并限定文章语言种类为PubMed、Cochrane、Web of Science和Embase。此外，在对文献进行筛选、质量评估和数据提取后，我们进行了荟萃分析。汇总受试者特征曲线(AUC)下的合并敏感性、特异性和相对危险度分别为0.98[95%可信区间(CI)：0.97~0.99]、0.99(95%CI：0.97~1.00)和1.00(95%CI：0.98~1.00)。CRISPR相关蛋白Cas-12的敏感性、特异性分别为0.98(95%CI：0.96~1.00)、1.00(95%CI：0.99~1.00)。对CAS13的敏感性和特异性分别为0.99(95%CI：0.97~1.00)和0.95(95%CI：0.91~1.00)。阳性似然比为183.2(95%CI：28.8,1166.8)，阴性似然比为0.02(95%CI：0.01，0.03)。RPA/RAA结合CRISPR技术用于新冠肺炎冠状病毒病的诊断准确率高，可用于大规模人群筛查。
To evaluate the diagnostic value of recombinase polymerase/ aided amplification (RPA/RAA) integrated clustered regularly interspaced short palindromic repeats (CRISPR) in the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We searched relevant literature on CRISPR technology for COVID-19 diagnosis using "novel coronavirus", "clustered regularly interspaced short palindromic repeats" and "RPA/RAA" as subject terms in PubMed, Cochrane, Web of Science, and Embase databases. Further, we performed a meta-analysis after screening the literature, quality assessment, and data extraction. The pooled sensitivity, specificity and a rea under the summary receiver operator characteristic curve (AUC) were 0.98 [95% confidence interval (CI):0.97–0.99], 0.99 (95% CI: 0.97–1.00) and 1.00 (95% CI: 0.98–1.00), respectively. For CRISPR-associated (Cas) proteins-12, the sensitivity, specificity was 0.98 (95% CI: 0.96–1.00), 1.00 (95% CI: 0.99–1.00), respectively. For Cas13, the sensitivity and specificity were 0.99 (95% CI: 0.97–1.00) and 0.95 (95% CI: 0.91–1.00). The positive likelihood ratio (PLR) was 183.2 (95% CI: 28.8, 1166.8); the negative likelihood ratio (NLR) was 0.02 (95% CI: 0.01, 0.03). RPA/RAA integrated with CRISPR technology is used to diagnose coronavirus disease-19 (COVID-19) with high accuracy and can be used for large-scale population screening.
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DOI：
10.1002/advs.202001300
发表时间：
2020-10
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
--
作者：
Ma P;Meng Q;Sun B;Zhao B;Dang L;Zhong M;Liu S;Xu H;Mei H;Liu J;Chi T;Yang G;Liu M;Huang X;Wang X
通讯作者：
Wang X
影响因子：
14.8
作者：
Liu, Tina Y.;Knott, Gavin J.;Smock, Dylan C. J.;Desmarais, John J.;Son, Sungmin;Bhuiya, Abdul;Jakhanwal, Shrutee;Prywes, Noam;Agrawal, Shreeya;Derby, Maria Diaz de Leon;Switz, Neil A.;Armstrong, Maxim;Harris, Andrew R.;Charles, Emeric J.;Thornton, Brittney W.;Fozouni, Parinaz;Shu, Jeffrey;Stephens, Stephanie, I;Kumar, G. Renuka;Zhao, Chunyu;Mok, Amanda;Iavarone, Anthony T.;Escajeda, Arturo M.;McIntosh, Roger;Kim, Shin E.;Dugan, Eli J.;Pollard, Katherine S.;Tan, Ming X.;Ott, Melanie;Fletcher, Daniel A.;Lareau, Liana F.;Hsu, Patrick D.;Savage, David F.;Doudna, Jennifer A.
通讯作者：
Doudna, Jennifer A.
影响因子：
16.6
作者：
Ding X;Yin K;Li Z;Lalla RV;Ballesteros E;Sfeir MM;Liu C
通讯作者：
Liu C
影响因子：
12.6
作者：
Lee CY;Degani I;Cheong J;Lee JH;Choi HJ;Cheon J;Lee H
通讯作者：
Lee H
影响因子：
6.1
作者：
Li S;Huang J;Ren L;Jiang W;Wang M;Zhuang L;Zheng Q;Yang R;Zeng Y;Luu LDW;Wang Y;Tai J
通讯作者：
Tai J