The immunogenic and pathogenic potential of short poly(C) tract Mengo viruses.
The immunogenic and pathogenic potential of short poly(C) tract Mengo viruses.
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短聚 (C) 链 Mengo 病毒的免疫原性和致病潜力。
DOI：
10.1006/viro.1996.0485
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Palmenberg,AC
中科院分区：
文献类型：
--
作者：
Osorio,JE;Martin,LR;Palmenberg,AC
We have shown previously that genetically engineered Mengo viruses with artificial deletions in their 5′ noncoding polyribocytidylic acid (poly(C)) tracts are highly attenuated for the natural murine host and also for other animals such as baboons, macaques, and domestic pigs. The present report further characterizes select short poly(C) tract Mengo viruses in the natural murine host. A positive correlation was found between the length of the poly(C) tract and murine virulence, as measured by virus brain titers and brain lesion scores after infection. Histological examination of brain tissue collected from infected animals clearly showed that the short poly(C) tract viruses did not induce the devastating pathological effects characteristic of animals inoculated with wild-type virus. Instead, the short-tract Mengo viruses proved excellent immunological agents. A dose of only 100 plaque-forming units of vMC24(poly(C) tract: C13UC10), injected subcutaneously, protected 80% of recipient animals against a normally lethal dose of encephalomyocarditis virus. The protection was long-lived, and animals similarly immunized with vMC0virus (poly(C) tract: C0) still had protective neutralizing antibody titers up to 16 months after inoculation. In addition, the short-tract viruses proved genetically stable, in that the vMC24virus did not yield detectable pathogenic revertants even after multiple, forced passages in 4-week-old mice. These studies suggest that Mengo viruses containing deletions in their poly(C) tracts are biologically safe and potent immunogens and imply that they may have uses as cardiovirus vaccines.
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DOI：
10.1007/978-3-7091-9326-6_8
发表时间：
1994
期刊：
Archives of virology. Supplementum
影响因子：
--
作者：
Palmenberg,AC;Osorio,JE
通讯作者：
Osorio,JE
影响因子：
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作者：
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通讯作者：
FELLNER, P
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DOI：
--
发表时间：
1962
期刊：
影响因子：
--
作者：
J. Gainer
通讯作者：
J. Gainer
影响因子：
3.7
作者：
R. Perez;M. Gander
通讯作者：
M. Gander
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影响因子：
14.9
作者：
D. Black;P. Stephenson;D. Rowlands;F. Brown
通讯作者：
F. Brown