Interactions between Candida albicans and Enterococcus faecalis in an Organotypic Oral Epithelial Model.
Interactions between Candida albicans and Enterococcus faecalis in an Organotypic Oral Epithelial Model.
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DOI：
10.3390/microorganisms8111771
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发表时间：
2020-11-11
期刊：
影响因子：
4.5
通讯作者：
Neelakantan P
中科院分区：
文献类型：
--
作者：
Krishnamoorthy AL;Lemus AA;Solomon AP;Valm AM;Neelakantan P
白色念珠菌作为一种机会致病菌，利用宿主的免疫系统，引起各种危及生命的感染。这种真菌的多态性使其具有巨大的优势，可以突破粘膜屏障，引起口腔和播散性感染。类似于C。白色念珠菌、粪肠球菌是一种主要的机会致病菌，在免疫功能低下的患者中引起严重关注。越来越多的证据表明，E。faecalis与C.白色念珠菌在人体内的疾病样本。虽然这两种生物体之间的相互作用概况已在非生物基质和小鼠模型上进行了研究，但它们在人类口腔粘膜表面上的相互作用研究尚不存在。在这里，我们第一次全面地描述了实验室和临床分离的C。白念珠菌（SC 5314和BF 1）和E.粪肠球菌（OG1RF和P52S）的器官型口腔粘膜模型。我们的研究结果表明，双物种生物膜导致深刻的表面侵蚀，并显着增加微生物侵入粘膜隔室，相比任何一个物种单独。值得注意的是，C.在E.粪便。相比之下，E.涉及群体感应、生物膜形成、毒力和哺乳动物细胞侵袭的粪菌基因下调。本研究强调了E. faecalis和C.粘膜组织中有白色念珠菌浸润。
Candida albicans as an opportunistic pathogen exploits the host immune system and causes a variety of life-threatening infections. The polymorphic nature of this fungus gives it tremendous advantage to breach mucosal barriers and cause oral and disseminated infections. Similar to C. albicans, Enterococcus faecalis is a major opportunistic pathogen, which is of critical concern in immunocompromised patients. There is increasing evidence that E. faecalis co-exists with C. albicans in the human body in disease samples. While the interactive profiles between these two organisms have been studied on abiotic substrates and mouse models, studies on their interactions on human oral mucosal surfaces are non-existent. Here, for the first time, we comprehensively characterized the interactive profiles between laboratory and clinical isolates of C. albicans (SC5314 and BF1) and E. faecalis (OG1RF and P52S) on an organotypic oral mucosal model. Our results demonstrated that the dual species biofilms resulted in profound surface erosion and significantly increased microbial invasion into mucosal compartments, compared to either species alone. Notably, several genes of C. albicans involved in tissue adhesion, hyphal formation, fungal invasion, and biofilm formation were significantly upregulated in the presence of E. faecalis. By contrast, E. faecalis genes involved in quorum sensing, biofilm formation, virulence, and mammalian cell invasion were downregulated. This study highlights the synergistic cross-kingdom interactions between E. faecalis and C. albicans in mucosal tissue invasion.
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影响因子：
3
作者：
Seneviratne, C. J.;Silva, W. J.;Samaranayake, L. P.
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影响因子：
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