A naturally occurring InDel variation in BraA.FLC.b (BrFLC2) associated with flowering time variation in Brassica rapa.
A naturally occurring InDel variation in BraA.FLC.b (BrFLC2) associated with flowering time variation in Brassica rapa.
复制标题
DOI：
10.1186/1471-2229-12-151
复制
发表时间：
2012-08-28
影响因子：
5.3
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Wu J;Wei K;Cheng F;Li S;Wang Q;Zhao J;Bonnema G;Wang X
开花期是油菜作物的一项重要性状。Floering Locus C(FLC)是一种MADS盒转录因子，对开花起到了很强的抑制作用。当植物暴露在低温下时，FLC的表达被沉默，从而激活了开花。白菜型油菜中有四份FLC。对不同分离群体的分析表明，BraA.Flc.a(BrFLC1)和BraA.Flc.b(BrFLC2)在控制白菜开花时间方面起主要作用。我们对9个油菜品种的BrFLC2序列进行了分析，发现在外显子4和内含子4之间存在一个57bp的插入/缺失(Indel)，导致一个非功能等位基因。总体而言，该突变的BrFLC2等位基因被鉴定为三种类型的转录本。Indel被用来开发基于PCR的标记，该标记被用来筛选159份白菜种质。缺失型仅存在于油菜型白菜中，包括SSP。Oleifera和SSP.Tricolaris，在其他亚种中不存在。缺失型与开花时间变异显著相关。相反，已报道的BrFLC1剪接位点变异也导致了一个非功能位点，但在油型油菜中检测到了剪接位点变异，但与开花时间的变异无关，尽管它与蔬菜类型油菜的开花时间变异有关。我们的结果表明，BrFLC2基因第4外显子和第4内含子之间自然发生的缺失突变是油菜开花时间变异的重要原因。BrFLC_1和BrFLC_2与开花时间变异的不同关系表明，油型和植物型油菜对开花时间的控制已分别进化。
Flowering time is an important trait in Brassica rapa crops. FLOWERING LOCUS C (FLC) is a MADS-box transcription factor that acts as a potent repressor of flowering. Expression of FLC is silenced when plants are exposed to low temperature, which activates flowering. There are four copies of FLC in B. rapa. Analyses of different segregating populations have suggested that BraA.FLC.a (BrFLC1) and BraA.FLC.b (BrFLC2) play major roles in controlling flowering time in B. rapa. We analyzed the BrFLC2 sequence in nine B. rapa accessions, and identified a 57-bp insertion/deletion (InDel) across exon 4 and intron 4 resulting in a non-functional allele. In total, three types of transcripts were identified for this mutated BrFLC2 allele. The InDel was used to develop a PCR-based marker, which was used to screen a collection of 159 B. rapa accessions. The deletion genotype was present only in oil-type B. rapa, including ssp. oleifera and ssp. tricolaris, and not in other subspecies. The deletion genotype was significantly correlated with variation in flowering time. In contrast, the reported splicing site variation in BrFLC1, which also leads to a non-functional locus, was detected but not correlated with variation in flowering time in oil-type B. rapa, although it was correlated with variation in flowering time in vegetable-type B. rapa. Our results suggest that the naturally occurring deletion mutation across exon 4 and intron 4 in BrFLC2 gene contributes greatly to variation in flowering time in oil-type B. rapa. The observed different relationship between BrFLC1 or BrFLC2 and flowering time variation indicates that the control of flowering time has evolved separately between oil-type and vegetable-type B. rapa groups.
登录
查看更多内容
影响因子：
11.6
作者：
Lou, Ping;Wu, Jian;McClung, C. Robertson
通讯作者：
McClung, C. Robertson
影响因子：
6.2
作者：
Kim, Soo-Yun;Park, Beom-Seok;Lee, Yeon-Hee
通讯作者：
Lee, Yeon-Hee
影响因子：
10.5
作者：
Searle, I;He, YH;Coupland, G
通讯作者：
Coupland, G
影响因子：
56.9
作者：
Johanson, U;West, J;Dean, C
通讯作者：
Dean, C
影响因子：
7.2
作者：
KOORNNEEF, M;BLANKESTIJNDEVRIES, H;PEETERS, T
通讯作者：
PEETERS, T