Translocations and amplification of the BCL2 gene are detected in interphase nuclei of non-Hodgkin's lymphoma by in situ hybridization with yeast artificial chromosome clones.
Translocations and amplification of the BCL2 gene are detected in interphase nuclei of non-Hodgkin's lymphoma by in situ hybridization with yeast artificial chromosome clones.
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通过与酵母人工染色体克隆的原位杂交，在非霍奇金淋巴瘤的间期细胞核中检测到 BCL2 基因的易位和扩增。
作者：
M. Taniwaki;G. A. Sliverman;K. Nishida;S. Horiike;S. Misawa;C. Shimazaki;I. Miura;M. Nagai;M. Abe;S. Fukuhara
利用酵母人工染色体（YACs），通过荧光原位杂交（FISH），在中期扩散和间期核中特异性检测携带t（14;18）的B细胞恶性肿瘤中BCL 2基因的易位和来自非霍奇金淋巴瘤（NHL）的细胞系（HBL-2）中BCL 2基因的扩增。将含有BCL 2基因yA 153 A6的YAC克隆用于单色FISH分析，所述YAC克隆是从BCL 2外显子1上游的约60 kb跨越到次要断裂点簇区域的约60 kb 3'的360-kb克隆。对7例NHL患者和1例急性淋巴细胞白血病患者进行了BCL 2易位分析。NHL患者间期核与yA 153 A6探针杂交显示3种信号。这是预期的，因为YAC克隆跨越18q21.3上的BCL 2断点区域。在1例急性淋巴细胞白血病患者中，用yA 153 A6探针进行的单色FISH在der（14）条带14q32.33处检测到BCL 2阳性信号，而在der（18）上未检测到信号。在HBL-2细胞系中，在特征性异常染色体18，add（18）（q23）上观察到BCL 2扩增;该区域荧光信号的周期性模式提示存在扩增子。使用含有更多着丝粒18q21.3基因胃泌素释放肽（y302 F10）和14q32.33基因（IgH; Y 6）的YAC克隆的双色FISH，我们通过显示衍生染色体18上18q21.3和14q32.33条带的并列来检测t（14;18）。这些YAC克隆的间期FISH为诊断携带t（14;18）的B细胞恶性肿瘤提供了一种快速方法。此外，我们还表明可以在单细胞水平上检测到BCL 2基因的易位和扩增。
Translocation of the BCL2 gene in B-cell malignancies carrying t(14;18) and amplification of the BCL2 gene in a cell line (HBL-2) derived from a non-Hodgkin's lymphoma (NHL) were detected specifically in both metaphase spreads and interphase nuclei by fluorescence in situ hybridization (FISH) using yeast artificial chromosomes (YACs). A YAC clone containing the BCL2 gene yA153A6, a 360-kb clone spanning from approximately 60 kb upstream of BCL2 exon 1 to approximately 60 kb 3' of the minor breakpoint cluster region, was used for single-color FISH analysis. Seven patients with NHL and one patient with acute lymphoblastic leukemia were analyzed for BCL2 translocations. Interphase nuclei of NHL patients showed three signals when hybridized with the yA153A6 probe. This was expected because the YAC clone spans the BCL2 breakpoint regions on 18q21.3. In a patient with acute lymphoblastic leukemia, a positive signal for BCL2 was detected on der(14) at band 14q32.33 by single-color FISH with the yA153A6 probe, whereas no signals were detected on der(18). The amplification of BCL2 in the HBL-2 cell line was observed on a characteristic abnormal chromosome 18, add(18)(q23); the periodic pattern of the fluorescent signal of this region was suggestive of an amplicon. Using double-color FISH with YAC clones containing the more centromeric 18q21.3 gene gastrin-releasing peptide (y302F10) and the 14q32.33 gene (IgH; Y6), we detected t(14;18) by showing the juxtaposition of the 18q21.3 and 14q32.33 bands on the derivative chromosome 18. Interphase FISH with these YAC clones provided a rapid procedure for the diagnosis of B-cell malignancies carrying t(14;18). In addition, we showed that translocations and amplification of the BCL2 gene can be detected at the single-cell level.
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影响因子：
56.9
作者：
TSUJIMOTO, Y;FINGER, LR;CROCE, CM
通讯作者：
CROCE, CM
影响因子：
56.9
作者：
TSUJIMOTO, Y;COSSMAN, J;CROCE, CM
通讯作者：
CROCE, CM
DOI：
10.1073/pnas.82.21.7439
发表时间：
1985-01-01
影响因子：
11.1
作者：
CLEARY, ML;SKLAR, J
通讯作者：
SKLAR, J
影响因子：
158.5
作者：
WEISS, LM;WARNKE, RA;CLEARY, ML
通讯作者：
CLEARY, ML
酵母人工染色体克隆染色体带 18q21 内的 2 兆碱基大小的重叠群在 BCL2 和纤溶酶原激活剂抑制剂 2 型之间建立了物理联系。
影响因子：
4.4
作者：
Silverman,GA;Jockel,JI;Domer,PH;Mohr,RM;Taillon-Miller,P;Korsmeyer,SJ
通讯作者：
Korsmeyer,SJ