A transcriptional timetable of autumn senescence.
A transcriptional timetable of autumn senescence.
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DOI：
10.1186/gb-2004-5-4-r24
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发表时间：
2004
期刊：
影响因子：
12.3
通讯作者：
Nilsson P
中科院分区：
文献类型：
--
作者：
Andersson A;Keskitalo J;Sjödin A;Bhalerao R;Sterky F;Wissel K;Tandre K;Aspeborg H;Moyle R;Ohmiya Y;Bhalerao R;Brunner A;Gustafsson P;Karlsson J;Lundeberg J;Nilsson O;Sandberg G;Strauss S;Sundberg B;Uhlen M;Jansson S;Nilsson P
利用杨树36354个est序列构建了一个微阵列，用于研究白杨叶片的秋季衰老。我们已经开发了基因组工具，使杨树属（白杨和白杨）被开发为研究树木生物学基本方面的全功能模型。我们已经进行了大规模的表达序列标签（EST）测序项目，并创建了具有显著基因覆盖的杨树微阵列。植物生物学中不能在一年生植物中研究的一个重要方面是参与诱导秋叶衰老的基因活性。基于从7个cDNA文库中获得的36354个杨树ESTs，我们建立了一个由13490个克隆组成的DNA微阵列。其中12376个（92%）克隆经重测序确认，所有序列均进行了注释和功能分类。在这里，我们使用微阵列研究了瑞典北部一棵自由生长的白杨树（Populus tremula）在自然秋季衰老期间的叶片转录物丰度。在13490个斑点克隆中，3792个基因在7个研究椰枣的所有叶片样本中都有显著表达。我们观察到基因表达的重大转变，与大量叶绿素降解相一致，这反映了从光合能力到线粒体呼吸、脂肪酸氧化和营养动员产生能量的转变。秋季的衰老与一年生植物的衰老有许多共同之处；例如，许多蛋白酶被诱导。我们还发现，在出现明显的衰老迹象之前，转录活性增加的证据，可能是为叶片的成分降解做准备。
A microarray has been created from 36,354 ESTs from Populus species and used to study autumn senescence in the leaves of the aspen tree Populus tremula. We have developed genomic tools to allow the genus Populus (aspens and cottonwoods) to be exploited as a full-featured model for investigating fundamental aspects of tree biology. We have undertaken large-scale expressed sequence tag (EST) sequencing programs and created Populus microarrays with significant gene coverage. One of the important aspects of plant biology that cannot be studied in annual plants is the gene activity involved in the induction of autumn leaf senescence. On the basis of 36,354 Populus ESTs, obtained from seven cDNA libraries, we have created a DNA microarray consisting of 13,490 clones, spotted in duplicate. Of these clones, 12,376 (92%) were confirmed by resequencing and all sequences were annotated and functionally classified. Here we have used the microarray to study transcript abundance in leaves of a free-growing aspen tree (Populus tremula) in northern Sweden during natural autumn senescence. Of the 13,490 spotted clones, 3,792 represented genes with significant expression in all leaf samples from the seven studied dates. We observed a major shift in gene expression, coinciding with massive chlorophyll degradation, that reflected a shift from photosynthetic competence to energy generation by mitochondrial respiration, oxidation of fatty acids and nutrient mobilization. Autumn senescence had much in common with senescence in annual plants; for example many proteases were induced. We also found evidence for increased transcriptional activity before the appearance of visible signs of senescence, presumably preparing the leaf for degradation of its components.
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DOI：
10.1073/pnas.091062498
发表时间：
2001-04-24
影响因子：
11.1
作者：
Tusher, VG;Tibshirani, R;Chu, G
通讯作者：
Chu, G
DOI：
10.1073/pnas.95.22.13330
发表时间：
1998-10-27
影响因子：
11.1
作者：
Sterky, F;Regan, S;Lundeberg, J
通讯作者：
Lundeberg, J
影响因子：
7.2
作者：
Finnemann, J;Schjoerring, JK
通讯作者：
Schjoerring, JK
影响因子：
7.4
作者：
KROL, M;SPANGFORT, MD;JANSSON, S
通讯作者：
JANSSON, S
影响因子：
7.4
作者：
KAMACHI, K;YAMAYA, T;OJIMA, K
通讯作者：
OJIMA, K