Cloning and Expression of an Anther-Abundant Polygalacturonase Gene BcMF17 from Brassica Campestris ssp. Chinensis
Cloning and Expression of an Anther-Abundant Polygalacturonase Gene BcMF17 from Brassica Campestris ssp. Chinensis
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甘蓝花药丰富的多聚半乳糖醛酸酶基因 BcMF17 的克隆和表达。
DOI：
10.1007/s11105-011-0298-1
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发表时间：
2011-04
影响因子：
2.1
通讯作者：
Cao J.
中科院分区：
文献类型：
--
作者：
Zhang A.;Chen Q.;Huang L.;Qiu L.;Cao J.
从油菜中克隆了一个多聚半乳糖醛酸酶基因，命名为BcMF 17（Brassica campestris Male Fertility 17）。该基因编码多聚半乳糖醛酸酶（PG），由1，194 bp开放阅读框沿着4个外显子和3个内含子组成，与BcMF 9具有79%的相似性（Huang et al. 2009）。详细的多氨基酸序列比对和系统发育树分析表明，BcMF 17属于一个花粉分支，可能参与花粉壁的形成。与芸苔属同源序列的多重比对表明BcMF 17在该属中高度保守。实时荧光定量PCR结果表明，BcMF 17基因的转录本主要在生殖组织中表达，在雄花组织中表达量最高，而在营养组织中表达量较低。BcMF 17在雄花组织中的转录本从花药原基期一直持续到成熟花粉期。BcMF 17在花药发育过程中原位杂交信号非常强。在花药发育过程中，BcMF 17的表达模式与BcMF 9的表达模式非常相似;然而，与BcMF 9不同的是，BcMF 17在此期间持续表达，并且在整个植物的生活史中检测到其转录本。
A polygalacturonase gene, designated BcMF17 (Brassica campestris Male Fertility 17), has been cloned from Brassica campastris. This gene, encoding polygalacturonase (PG), consists of a 1,194 bp open reading frame along with four exons and three introns, and exhibits 79% similarities to BcMF9 (Huang et al. 2009). Detailed multiple amino acid sequence alignment and phylogenetic tree analysis indicate that BcMF17 belongs to a pollen clade and may be involved in pollen wall formation. Multiple alignments against homologous sequences from the genus Brassica indicate that BcMF17 is highly conserved in this genus. Quantitative real-time PCR results indicate that transcripts of BcMF17 are preferentially expressed in reproductive tissues, especially abundant in male floral tissues, but are expressed at low level in vegetative tissues. BcMF17 transcripts in male floral tissues persist from the anther primordia stage till to the mature pollen stage. In situ hybridized signals of BcMF17 are highly detected during anther development. During anther development, expression patterns of BcMF17 are very similar to those of BcMF9; however, unlike BcMF9, BcMF17 is continuously expressed during this period, and its transcripts are detected in the whole plant during its life cycle.
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影响因子：
--
作者：
X. Qiu;L. Erickson
通讯作者：
X. Qiu;L. Erickson
影响因子：
2.1
作者：
Jian Wang;N. Tian;Xuan Huang;Li Yu Chen;M. Schläppi;Ziqin Xu
通讯作者：
Jian Wang;N. Tian;Xuan Huang;Li Yu Chen;M. Schläppi;Ziqin Xu
影响因子：
5.1
作者：
L. Robert;S. Allard;J. Gerster;L. Cass;J. Simmonds
通讯作者：
L. Robert;S. Allard;J. Gerster;L. Cass;J. Simmonds
影响因子：
3.2
作者：
Campbell Louw;Philip R. Young;P. van Rensburg;B. Divol
通讯作者：
Campbell Louw;Philip R. Young;P. van Rensburg;B. Divol
影响因子：
3.1
作者：
K.-C. Park;S.-J. Kwon;P-H Kim;T. Bureau;N.-S. Kim
通讯作者：
K.-C. Park;S.-J. Kwon;P-H Kim;T. Bureau;N.-S. Kim