Sequential changes of extracellular matrix and proliferation of Ito cells with enhanced expression of desmin and actin in focal hepatic injury.
Sequential changes of extracellular matrix and proliferation of Ito cells with enhanced expression of desmin and actin in focal hepatic injury.
复制标题
局灶性肝损伤中细胞外基质和 Ito 细胞增殖的连续变化以及结蛋白和肌动蛋白表达的增强。
作者：
K. Ogawa;J. Suzuki;H. Mukai;M. Mori
对局灶性肝损伤中细胞和细胞外基质（ECM）的特性进行了免疫组织化学研究。将液氮冷却的注射器针头刺入大鼠肝脏。坏死区域在 24 小时内就充满了血浆。纤连蛋白区域呈强阳性，并被溶菌酶呈阳性的炎症细胞浸润。到了第三天，受损区域的周边部分的伊藤细胞开始增殖。这些 Ito 细胞显示结蛋白和肌动蛋白的免疫染色增强，但溶菌酶呈阴性。从第3天起，I型和IV型胶原、层粘连蛋白和纤连蛋白免疫化学呈阳性的间质纤维开始增加。它们出现在邻近受损区域的肝细胞边缘，并与Ito细胞一起延伸到受损区域。与毛细血管和胆管相关的基底层的增加也随着 1 天的延迟而增加。第5天，受损区域被肉芽组织取代。随后肉芽组织迅速减少，7-10天后恢复正常肝组织。这些观察结果表明，ECM 以连续的方式发生变化，最终在 10 天内从受损部位消失。尽管包括实质细胞、巨噬细胞、内皮细胞和胆管细胞在内的各种细胞都有助于受损区域的愈合，但表现出独特表型变化的伊藤细胞可能在此过程中发挥了重要作用。
Immunohistochemical investigations were carried out on the properties of the cells and extracellular matrix (ECM) in focal hepatic injuries. A liquid nitrogen-cooled syringe needle was thrust into the rat liver. Necrotic areas became permeated with plasma within 24-hour period. Areas became strongly positive for fibronectin and were infiltrated with inflammatory cells positive for lysozyme. By the third day, Ito cells were proliferated in the peripheral portions of the damaged areas. These Ito cells showed enhanced immunostaining for desmin and actin but were negative for lysozyme. Interstitial fibers which were immunochemically positive for Types I and IV collagens, laminin, and fibronectin, began to increase from Day 3. They appeared on the rim of the hepatocytes adjacent to the damaged areas and extended into the injured regions with the Ito cells. An increase in basal laminas associated with capillaries and bile ducts also increased with a 1-day delay. The damaged areas were replaced by granulation tissue by Day 5. A rapid diminution then occurred in the granulation tissue, and normal hepatic tissue was restored in 7-10 days. These observations demonstrate that ECM changed in a sequential manner and then finally disappeared from the damaged site within 10 days. Although various cells, including parenchymal cells, macrophages, endothelial cells, and cholangiolar cells contributed to the healing of the damaged area, Ito cells, which exhibit unique phenotypic changes, presumably had a major role in the process.
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