Transmigration of human neutrophils across airway epithelial cell monolayers is preferentially in the physiologic basolateral-to-apical direction.
Transmigration of human neutrophils across airway epithelial cell monolayers is preferentially in the physiologic basolateral-to-apical direction.
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人中性粒细胞穿过气道上皮细胞单层的迁移优先沿生理基底外侧到顶端方向。
DOI：
10.1165/ajrcmb.15.6.8969272
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发表时间：
1996
影响因子：
6.4
通讯作者：
E. Knol
中科院分区：
文献类型：
--
作者：
L. Liu;F. Mul;R. Lutter;D. Roos;E. Knol
为了研究中性粒细胞从血流进入肺气道的运动机制，我们研究了人中性粒细胞在单层人气道上皮细胞中的迁移，包括顶侧至基底侧方向和更生理性的基底侧至顶侧方向。在向静息上皮细胞添加化学引诱剂后，以及在白细胞介素-1 β（IL-1 β）刺激的上皮细胞中，人中性粒细胞在人气道上皮H292细胞系细胞单层和原代支气管上皮细胞单层中的迁移最有效地发生在基底外侧至顶端方向。单克隆抗体阻断研究表明，中性粒细胞的迁移是由中性粒细胞上的CR 3粘附分子（CD 11b/CD 18）介导的。IL-1 β处理的上皮细胞通过分泌化学引诱物引起中性粒细胞运动。发现上皮细胞释放的最有效的化学引诱物是IL-8，因为IL-1 β诱导的迁移被添加抗IL-8的抗体抑制了75 +/- 10%。用最佳浓度的IL-1 β刺激上皮细胞顶端后，在上皮细胞顶端的上清液中发现了27 +/- 4 ng/ml的IL-8。上皮细胞的血小板活化因子（PAF）合成在中性粒细胞迁移中不起作用，如加入PAF受体拮抗剂WEB 2086后该过程缺乏抑制所证明的那样。我们的结论是，在气道上皮细胞单层的中性粒细胞的运动优先发生在生理的基底侧向顶端方向，表明上皮细胞的极性是重要的中性粒细胞的迁移。
To study the mechanisms involved in the movement of neutrophils from the blood stream into the lung airways, we investigated human neutrophil transmigration across a monolayer of human airway epithelial cells, both in the apical-to-basolateral direction and in the more physiologic basolateral-to-apical direction. Migration of human neutrophils across monolayers of human airway epithelial H292 cell-line cells and primary bronchial epithelial cells occured most efficiently in the basolateral-to-apical direction, both after the addition of chemoattractants to resting epithelial cells and across interleukin-1beta (IL-1beta)-stimulated epithelial cells. Blocking studies with monoclonal antibodies revealed that the migration of neutrophils was mediated by the CR3 adhesion molecule (CD11b/CD18) on the neutrophils. IL-1beta-treated epithelial cells caused neutrophil movement via the secretion of chemoattractants. The most potent chemoattractant released by the epithelial cells was found to be IL-8, because the IL-1beta-induced migration was inhibited for 75 +/- 10% by the addition of an antibody against IL-8. After apical stimulation of the epithelial cells with an optimal concentration of IL-1beta, 27 +/- 4 ng/ml IL-8 was found in the supernatant at the apical side of epithelial cells. Platelet-activating factor (PAF) synthesis by the epithelial cells did not play a role in neutrophil transmigration, as was demonstrated by the lack of inhibition of this process after addition of the PAF-receptor antagonist WEB 2086. We conclude that the movement of neutrophils across airway epithelial cell monolayers occurs preferentially in the physiologic basolateral-to-apical direction, indicating that the polarity of epithelial cells is important for neutrophil transmigration.
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