Molecular characterization of a new Trypanosoma (Megatrypanum) theileri isolate supports the two main phylogenetic lineages of this species in Japanese cattle
Molecular characterization of a new Trypanosoma (Megatrypanum) theileri isolate supports the two main phylogenetic lineages of this species in Japanese cattle
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一种新的锥虫 (Megatrypanum) theileri 分离物的分子特征支持该物种在日本牛中的两个主要系统发育谱系
DOI：
10.1007/s00436-019-06313-x
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发表时间：
2019
影响因子：
2
通讯作者：
Inoue Noboru
中科院分区：
文献类型：
--
作者：
Suganuma Keisuke;Kondoh Daisuke;Sivakumar Thillaiampalam;Mizushima Daiki;Elata Afra’a Tajelsir Mohamed;Thekisoe Oriel M. M.;Yokoyama Naoaki;Inoue Noboru
泰勒氏锥虫是一种世界性的牛锥虫，通常是非致病性的，由吸血节肢动物传播，主要是蝇科昆虫。SeveralT。通过与哺乳动物饲养细胞或血细胞共培养，从家畜和野生反刍动物中分离到的泰勒氏杆菌已经在形态和遗传学上进行了表征。在这里，我们从日本北海道的一头荷斯坦奶牛身上培养了一株新的锥虫分离物，并进行了形态和分子特征研究。新分离株(Obihiro株)与Madin-Darby牛肾(MDBK)细胞在添加10%胎牛血清的GIT培养液中共培养。在培养物中发现了锥形鞭毛虫和上鞭毛虫，但没有发现细胞内寄生虫。对18S rRNA序列的V7-V8区的分析表明，Obihiro株位于巨藻亚属内，基于整个内部转录间隔区rDNA序列的树状图显示Obihiro株与日本分离株Oft一起聚在TthII谱系中。Theileri、Esashi 9和Esashi 12，以及从赞比亚和美国分离到的T.theileri Km菌株和At.从鹿(TSD1品系)分离的泰勒里样锥虫聚集在TthI谱系中，与Obihiro品系分开。基于部分组织蛋白酶L样蛋白基因分析，Obihiro菌株与TthIIF基因型的分离物聚在一起，其中包括T。他们来自越南、斯里兰卡和巴西。我们对Thet的分析。他们的Obihiro株对其在日本牛中的遗传多样性提供了相关的见解，并证实了巨型锥虫亚属的牛和鹿锥虫的宿主特异性。
Trypanosoma (Megatrypanum) theileriis a cosmopolitan, usually non-pathogenic, trypanosome of cattle transmitted by blood-sucking arthropods, mainly tabanid flies. SeveralT. theileristrains isolated from domestic and wild ruminants via co-culturing with mammalian feeder cells or blood cells have been characterized morphologically and genetically. Here, we cultured a new trypanosome isolate from a Holstein cow in Hokkaido, Japan, and performed morphological and molecular characterization studies. The new isolate (Obihiro strain) was co-cultivated with Madin–Darby bovine kidney (MDBK) cells in GIT medium supplemented with 10% fetal bovine serum. Trypomastigotes and epimastigotes, but not intracellular parasites, were identified in the culture. Analysis of the V7-V8 region of18S rRNAsequences showed that the Obihiro strain is positioned within the subgenusMegatrypanum.A dendrogram based on whole internal transcribed spacer rDNA sequence showed that the Obihiro strain clustered in the lineage TthII together with the Japanese isolates ofT. theileri, Esashi 9, and Esashi 12, and isolates from Zambia and the USA.T. theileriof the KM strain and aT. theileri-like trypanosome isolated from deer (TSD1 strain) clustered in the lineage TthI, separate from the Obihiro strain. Based on a partial cathepsin L-like protein gene analysis, the Obihiro strain clustered with isolates of the TthIIF genotype, which includesT. theilerifrom Vietnam, Sri Lanka, and Brazil. Our analyses of theT. theileriObihiro strain provide relevant insights into its genetic diversity in Japanese cattle and corroborate the host specificity of cattle and deer trypanosomes of the subgenusMegatrypanum.
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影响因子：
1.3
作者：
KINGSTON, N;MORTON, JK
通讯作者：
MORTON, JK
从台湾奶牛中分离出特氏锥虫（Megatrypanum）。
影响因子：
1.2
作者：
Yen;Ching;N. Lin;Shih;K. Tung;Y. Chiu
通讯作者：
Y. Chiu
影响因子：
2.6
作者：
Rodrigues, AC;Campaner, M;Teixeira, MMG
通讯作者：
Teixeira, MMG
影响因子：
2.6
作者：
Fisher, Amanda C.;Schuster, Greta;Holman, Patricia J.
通讯作者：
Holman, Patricia J.
非吞噬哺乳动物细胞中泰氏锥虫 (Megatrypanum) 细胞内阶段的证据。
影响因子：
2.6
作者：
Yen;Ching;Jiun;N. Lin;Y. Hung;Jiunn;W. Tu;K. Tung;Y. Chiu
通讯作者：
Y. Chiu