Guidelines for assessing mouse endothelial function via ultrasound imaging: a report from the International Society Of Cardiovascular Translational Research.
Guidelines for assessing mouse endothelial function via ultrasound imaging: a report from the International Society Of Cardiovascular Translational Research.
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DOI：
10.1007/s12265-015-9614-8
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发表时间：
2015-03
影响因子：
3.4
通讯作者：
Zhang, Chunxiang
中科院分区：
文献类型：
--
作者：
Wang, Hua-ting;Shan, Zhen;Li, Wen;Chu, Maoping;Yang, Jian;Yi, Dan;Zhan, Jiaxin;Yuan, Zu-Yi;Raikwar, Sudhanshu;Wang, Shenming;Zhang, Chunxiang
本研究旨在建立一种利用高分辨率超声图像在体检测小鼠颈动脉内皮功能的新方法。用西方饮食在ApoE−/−小鼠中诱导颈动脉粥样硬化。腹侧颈部的超声波可生成颈总动脉的清晰图像。乙酰胆碱在5 - 20 μg/kg/min（iv）范围内能够诱导剂量依赖性舒张，如这些正常小鼠颈动脉直径增加所示，其在动脉粥样硬化动脉中受损。在体内超声图像确定的内皮功能与在离体颈动脉环中确定的内皮功能匹配良好。内皮功能测定后所有动物均存活。在本研究中，我们建立了一个标准的方法来测定小鼠内皮功能在体内。该方法可靠、安全、成活率高，可在小鼠动脉中重复使用。
The study is to establish a novel method to determine the endothelial function in mouse carotid arteries in vivo by using high-resolution ultrasound images. Atherosclerosis in carotid arteries is induced in ApoE−/− mice with a Western diet. The ultrasound of the ventral neck generates clear pictures of the common carotid arteries. Acetylcholine at the range from 5 to 20 μg/kg/min (iv) is able to induce a dose-dependent relaxation as shown by the increased diameter of these normal mouse carotid arteries, which is impaired in atherosclerotic arteries. The endothelial function determined by ultrasound images in vivo matches well with that determined in isolated carotid arterial rings in vitro. All animals are survival after the endothelial function measurement. In this study, we have established a standard method to determine the mouse endothelial function in vivo. It is a reliable, safe and survival method that could be used repetitively in mouse arteries.
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影响因子：
0.7
作者：
Mudau M;Genis A;Lochner A;Strijdom H
通讯作者：
Strijdom H
DOI：
10.1161/atvbaha.109.188839
发表时间：
2009-07
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Wang H;Zhang W;Zhu C;Bucher C;Blazar BR;Zhang C;Chen JF;Linden J;Wu C;Huo Y
通讯作者：
Huo Y
影响因子：
2.8
作者：
Guo, YK;Zhang, CX;Yoo, TJ
通讯作者：
Yoo, TJ
影响因子：
4.6
作者：
Lockowandt, U;Liska, J;Franco-Cereceda, A
通讯作者：
Franco-Cereceda, A
DOI：
10.1093/gerona/gln049
发表时间：
2009-01-01
影响因子：
5.1
作者：
Lesniewski, Lisa A.;Connell, Melanie L.;Seals, Douglas R.
通讯作者：
Seals, Douglas R.