Genome-wide nucleosome specificity and directionality of chromatin remodelers.
Genome-wide nucleosome specificity and directionality of chromatin remodelers.
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DOI：
10.1016/j.cell.2012.04.036
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发表时间：
2012-06-22
期刊：
影响因子：
64.5
通讯作者：
Pugh BF
中科院分区：
文献类型：
--
作者：
Yen K;Vinayachandran V;Batta K;Koerber RT;Pugh BF
染色质重塑者如何合作组织核小体围绕基因的开始和结束是未知的。我们确定了重塑复合物SWI/SNF，RSC，ISW 1a，ISW 1b，ISW 2和INO 80与酵母中单个核小体的全基因组结合，并通过缺失分析确定了它们对核小体定位的功能贡献。我们将超高分辨率ChIP-exo映射应用于Isw 2，以确定其在基因组规模上的亚核小体方向和组织。重塑物与相对于基因起始和末端的选定核小体位置相互作用，并在将核小体移离或移向基因5′和3′末端的无核小体区域时产生净方向性。Isw 2具有亚核小体组织，这与基于生物化学和晶体学的模型雅阁，该模型将其接头结合区域置于启动子内，并与Reb 1结合的位置相反。总之，这些发现揭示了一个协调的位置特定的方法所采取的重塑组织基因核小体成阵列。
How chromatin remodelers cooperate to organize nucleosomes around the start and end of genes is not known. We determined the genome-wide binding of remodeler complexes SWI/SNF, RSC, ISW1a, ISW1b, ISW2, and INO80 to individual nucleosomes in Saccharomyces, and determined their functional contributions to nucleosome positioning through deletion analysis. We applied ultra-high resolution ChIP-exo mapping to Isw2 to determine its sub-nucleosomal orientation and organization on a genomic scale. Remodelers interacted with selected nucleosome positions relative to the start and end of genes, and produced net directionality in moving nucleosomes either away or towards nucleosome-free regions at the 5′ and 3′ ends of genes. Isw2 possessed a sub-nucleosomal organization in accord with biochemical and crystallographic-based models that place its linker binding region within promoters and abutted against Reb1-bound locations. Together these findings reveal a coordinated position-specific approach taken by remodelers to organize genic nucleosomes into arrays.
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影响因子：
16
作者：
Badis, Gwenael;Chan, Esther T.;van Bakel, Harm;Pena-Castillo, Lourdes;Tillo, Desiree;Tsui, Kyle;Carlson, Clayton D.;Gossett, Andrea J.;Hasinoff, Michael J.;Warren, Christopher L.;Gebbia, Marinella;Talukder, Shaheynoor;Yang, Ally;Mnaimneh, Sanie;Terterov, Dimitri;Coburn, David;Yeo, Ai Li;Yeo, Zhen Xuan;Clarke, Neil D.;Lieb, Jason D.;Ansari, Aseem Z.;Nislow, Corey;Hughes, Timothy R.
通讯作者：
Hughes, Timothy R.
影响因子：
30.8
作者：
Ioshikhes, Ilya P.;Albert, Istvan;Pugh, B. Franklin
通讯作者：
Pugh, B. Franklin
影响因子：
5.3
作者：
Gangaraju, Vamsi K.;Bartholomew, Blaine
通讯作者：
Bartholomew, Blaine
影响因子：
11.4
作者：
Kagalwala, MN;Glaus, BJ;Bartholomew, B
通讯作者：
Bartholomew, B
影响因子：
64.5
作者：
Hartley PD;Madhani HD
通讯作者：
Madhani HD