OVA-Experienced CD4(+) T Cell Transfer and Chicken Protein Challenge Affect the Immune Response to OVA in a Murine Model.
OVA-Experienced CD4(+) T Cell Transfer and Chicken Protein Challenge Affect the Immune Response to OVA in a Murine Model.
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DOI：
10.3390/ijms22126573
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发表时间：
2021-06-18
影响因子：
5.6
通讯作者：
Wróblewska B
中科院分区：
文献类型：
--
作者：
Fuc E;Złotkowska D;Wasilewska E;Wróblewska B
鸡肉通常是现代饮食的主要组成部分。对鸡肉过敏是相对罕见的，并且独立发生或在对卵清蛋白（OVA）过敏的受试者中发生。我们研究了过继转移OVA-CD 4 + T细胞对饲喂鸡肉的小鼠对OVA的免疫应答的影响。供体小鼠在一周内两次腹腔注射100 μg含有弗氏佐剂的OVA，并从中分离出CD 4 + T细胞并转移至未处理小鼠（CD 4 +/OVA/ChM组），然后用含有FA的OVA激发并喂食冻干鸡肉14天。以注射OVA并饲喂鸡肉的小鼠（OVA/ChM组）、致敏小鼠（OVA组）和健康小鼠（PBS组）作为对照。在研究期间监测对OVA的体液和细胞应答。CD 4 +/OVA/ChM组抗OVA IgG和伊加以及总IgE水平降低。组间CD 4+、CD 4 + CD 25+和CD 4 + CD 25 + Foxp 3 + T细胞存在显著差异。与OVA组相比，OVA刺激降低了CD 4 +/OVA/ChM组的脾细胞增殖指数和IFN-γ分泌。IL-4在OVA/ChM小鼠中增加，这证实了蛋-肉蛋白组合的致敏潜力。经OVA处理的CD 4 + T细胞的转移改善了对OVA的负性免疫应答。
Chicken meat is often a major component of a modern diet. Allergy to chicken meat is relatively rare and occurs independently or in subjects allergic to ovalbumin (OVA). We examined the effect of adoptive transfer of OVA-CD4+ T cells on the immune response to OVA in mice fed chicken meat. Donor mice were injected intraperitoneally with 100 µg of OVA with Freund’s adjuvant two times over a week, and CD4+ T cells were isolated from them and transferred to naïve mice (CD4+/OVA/ChM group), which were then provoked with OVA with FA and fed freeze-dried chicken meat for 14 days. The mice injected with OVA and fed chicken meat (OVA/ChM group), and sensitized (OVA group) and healthy (PBS group) mice served as controls. Humoral and cellular response to OVA was monitored over the study. The CD4+/OVA/ChM group had lowered levels of anti-OVA IgG and IgA, and total IgE. There were significant differences in CD4+, CD4+CD25+, and CD4+CD25+Foxp3+ T cells between groups. OVA stimulation decreased the splenocyte proliferation index and IFN-γ secretion in the CD4+/OVA/ChM group compared to the OVA group. IL-4 was increased in the OVA/ChM mice, which confirms allergenic potential of the egg–meat protein combination. Transfer of OVA-experienced CD4+ T cells ameliorated the negative immune response to OVA.
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DOI：
10.4103/0970-2113.63609
发表时间：
2010-04
期刊：
Lung India : official organ of Indian Chest Society
影响因子：
--
作者：
Deo SS;Mistry KJ;Kakade AM;Niphadkar PV
通讯作者：
Niphadkar PV
DOI：
10.1084/jem.20081571
发表时间：
2009-01-16
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Dienz O;Eaton SM;Bond JP;Neveu W;Moquin D;Noubade R;Briso EM;Charland C;Leonard WJ;Ciliberto G;Teuscher C;Haynes L;Rincon M
通讯作者：
Rincon M
影响因子：
14.2
作者：
Klug, Christoph;Hemmer, Wolfgang;Swoboda, Ines
通讯作者：
Swoboda, Ines
当前对鸡蛋过敏的理解。
影响因子：
2.6
作者：
Caubet, Jean-Christoph;Wang, Julie
通讯作者：
Wang, Julie
影响因子：
14.2
作者：
Hoffman, KM;Ho, DG;Sampson, HA
通讯作者：
Sampson, HA